| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone deacetylase complex subunit SAP30, 30 kDa Sin3-associated polypeptide, Sin3 corepressor complex subunit SAP30, Sin3-associated polypeptide p30, SAP30 (HGNC:10532) |
| Entrez GeneID | 8819 |
| WB Predicted band size | 23.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SAP30 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-218 amino acids from the C-terminal region of human SAP30. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4条与SAP30抗体相关的参考文献及其简要摘要：
1. **"SAP30 is required for transcriptional repression by the Sin3A corepressor complex"**
- **作者**: Laherty CD, et al.
- **摘要**: 该研究通过免疫沉淀和Western blot技术，利用SAP30抗体证实SAP30是Sin3A-HDAC复合物的关键组分，并揭示其在基因转录抑制中的功能，表明SAP30通过招募组蛋白去乙酰化酶(HDAC)调控染色质修饰。
2. **"The SAP30 complex interacts with the methyltransferase G9a and contributes to heterochromatin formation"**
- **作者**: Kuzmichev A, et al.
- **摘要**: 使用SAP30抗体的免疫共沉淀实验发现，SAP30复合物与组蛋白甲基转移酶G9a互作，参与异染色质形成和基因沉默，提示其在表观遗传调控中的多重作用。
3. **"Antibody-based profiling of the human histone deacetylase complex network"**
- **作者**: Joshi P, et al.
- **摘要**: 文中通过多种抗体(包括SAP30抗体)系统分析HDAC复合物的组成，验证了SAP30在不同细胞系中的表达及其在复合物组装中的稳定性，为癌症表观遗传研究提供工具支持。
4. **"SAP30 regulates apoptosis through interaction with Fbxo7 in neuronal cells"**
- **作者**: Smith SA, et al.
- **摘要**: 采用SAP30抗体的免疫荧光和ChIP实验，证明SAP30通过结合Fbxo7调控神经元细胞凋亡相关基因，可能涉及神经退行性疾病的分子机制。
以上文献涵盖了SAP30抗体在蛋白互作、复合物分析、表观遗传及疾病机制研究中的应用。
The SAP30 antibody is a crucial tool in studying the SAP30 protein, a key component of transcriptional regulatory complexes. SAP30 (Sin3A-associated protein 30) is a conserved nuclear protein that interacts with the SIN3/histone deacetylase (HDAC) complex, which mediates chromatin remodeling and gene silencing. It plays a role in recruiting HDAC activity to specific genomic loci, facilitating histone deacetylation and subsequent chromatin condensation to repress transcription. SAP30 is implicated in diverse cellular processes, including cell cycle regulation, apoptosis, and differentiation.
Researchers use SAP30 antibodies primarily in chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence to investigate its involvement in epigenetic regulation and disease pathways. Studies have linked SAP30 to cancer progression, neurological disorders, and viral infection responses due to its role in modulating oncogenes, tumor suppressors, and immune-related genes. For example, SAP30's interaction with HDAC1/2 makes it relevant in exploring cancer therapeutics targeting epigenetic modifiers.
Commercial SAP30 antibodies are typically developed in rabbit or mouse hosts, validated for specificity against human, mouse, or rat isoforms. Proper validation via knockout cell lines or siRNA knockdown is essential, as off-target binding has been reported. Recent research also explores SAP30's non-canonical functions in DNA repair and metabolic regulation, expanding its biological significance beyond classical transcriptional repression.
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