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Rabbit Polyclonal PAF1 Antibody

  • 中文名: PAF1抗体
  • 别    名: RNA polymerase II-associated factor 1 homolog, hPAF1, Pancreatic differentiation protein 2, PAF1, PD2
货号: IPDX31729
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesRNA polymerase II-associated factor 1 homolog, hPAF1, Pancreatic differentiation protein 2, PAF1, PD2
Entrez GeneID54623
WB Predicted band size60.0kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis PAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 439-467 amino acids from the C-terminal region of human PAF1.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于PAF1抗体的3篇代表性文献(虚拟示例,供参考):

1. **标题**:*"PAF1 Complex Subunit Characterization and Antibody Development for Epigenetic Studies"*

**作者**:Smith J, et al.

**摘要**:本研究开发了针对PAF1复合体核心亚基(包括PAF1、CDC73等)的高特异性多克隆抗体,验证了其在染色质免疫沉淀(ChIP)和免疫荧光中的应用,揭示了PAF1在组蛋白H2B单泛素化中的调控作用。

2. **标题**:*"PAF1 Antibody-Based Profiling Identifies Its Role in Transcriptional Elongation and Cancer"*

**作者**:Lee H, Kim T.

**摘要**:通过PAF1特异性抗体进行全基因组结合位点分析,发现PAF1复合体与RNA聚合酶II的转录延伸密切相关,且在多种癌症中异常高表达,提示其作为肿瘤标志物的潜力。

3. **标题**:*"Antibody Validation for PAF1 in Stem Cell Differentiation"*

**作者**:Garcia R, et al.

**摘要**:验证了商业化PAF1抗体的特异性,并应用于胚胎干细胞分化模型,证明PAF1通过调控关键多能性基因(如OCT4)的转录终止影响细胞命运决定。

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**说明**:实际文献需通过PubMed/Google Scholar检索关键词(如"PAF1 antibody validation"、"PAF1 complex ChIP")。推荐补充检索真实文献,例如:

- 关注PAF1复合体在转录或癌症中的机制的权威研究(如Nature、Cell系列期刊)。

- 抗体开发相关的技术论文(如《Journal of Biological Chemistry》)。

背景信息

The PAF1 (RNA Polymerase II-Associated Factor 1) antibody is a crucial tool for studying the PAF1 complex, a multi-protein assembly conserved from yeast to humans. This complex, comprising PAF1. LEO1. CTR9. RTF1. and CDC73. plays diverse roles in transcriptional regulation, including facilitating transcription elongation, coordinating histone modifications (e.g., H2B ubiquitination, H3K4 methylation), and linking transcription to mRNA processing. PAF1 itself is essential for complex stability and function. Antibodies targeting PAF1 enable researchers to investigate its interactions, localization, and expression levels in various biological contexts. Studies using PAF1 antibodies have revealed its involvement in stem cell maintenance, cell cycle progression, and DNA repair. Dysregulation of the PAF1 complex is implicated in cancers, developmental disorders, and other diseases, making these antibodies valuable for mechanistic studies and potential biomarker discovery. Common applications include chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence to dissect PAF1's role in gene expression networks. Recent work highlights PAF1's emerging connection to epigenetic regulation and cancer metastasis, driving ongoing interest in its functional characterization.

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