| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Drebrin-like protein, Cervical SH3P7, Cervical mucin-associated protein, Drebrin-F, HPK1-interacting protein of 55 kDa, HIP-55, SH3 domain-containing protein 7, DBNL, CMAP, SH3P7 |
| Entrez GeneID | 28988 |
| WB Predicted band size | 48.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This DBNL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 292-319 amino acids from the C-terminal region of human DBNL. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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1. **"DBNL regulates actin dynamics and coordinates with T-cell receptor signaling to control immune synapse organization"**
*Author: Smith A, et al.*
*摘要：研究揭示了DBNL蛋白通过调节T细胞受体信号传导中的肌动蛋白重组，影响免疫突触形成。文中使用DBNL抗体进行免疫沉淀和共聚焦成像，证实其与F-actin的相互作用。*
2. **"DBNL modulates glioblastoma cell migration by regulating filopodia formation via Cdc42 signaling"**
*Author: Chen L, et al.*
*摘要：通过Western blot和免疫荧光技术，发现DBNL抗体可特异性检测胶质母细胞瘤细胞中DBNL表达水平，并证明其通过Cdc42通路调控细胞伪足形成及侵袭能力。*
3. **"Characterization of DBNL knockout mice reveals its essential role in neuronal development"**
*Author: Tanaka R, et al.*
*摘要：利用DBNL抗体进行脑组织免疫组化分析，发现DBNL缺失导致小鼠海马区神经元树突棘发育异常，提示其在神经突触可塑性中的关键作用。*
4. **"DBNL interacts with WASP/WAVE proteins to coordinate endocytosis and actin remodeling"**
*Author: Müller S, et al.*
*摘要：通过免疫共沉淀(Co-IP)结合DBNL抗体的实验，证实DBNL与WASP家族蛋白形成复合物，调控网格蛋白介导的内吞过程及细胞骨架动态变化。*
注：以上文献信息为示例性质，实际文献需通过PubMed或专业数据库检索。建议使用关键词"DBNL antibody" "CTTNBP2"或"DBNL function"查找具体研究。
The DBNL (Drebrin-like) antibody targets the DBNL protein, a key adaptor molecule involved in regulating actin cytoskeleton dynamics and intracellular signaling pathways. DBNL, also known as SH3P7 or mAbp1. belongs to the actin-binding protein family and contains multiple functional domains, including an N-terminal actin-depolymerizing factor homology (ADF-H) domain and C-terminal SH3 domains. These structural features enable DBNL to interact with various partners such as WASP, N-WASP, and dynamin, facilitating its role in membrane trafficking, endocytosis, and immune synapse formation.
Discovered through its homology to drebrin, DBNL is ubiquitously expressed but shows particular importance in neuronal development and immune cell function. It participates in clathrin-mediated endocytosis by linking actin remodeling to vesicle formation and coordinates T-cell receptor clustering during immune responses. Studies using DBNL antibodies have revealed its involvement in pathological conditions, including neurodegenerative diseases and cancer metastasis, where cytoskeletal dysregulation occurs. Researchers employ DBNL-specific antibodies primarily in Western blotting, immunofluorescence, and co-immunoprecipitation experiments to investigate its expression patterns, subcellular localization, and protein interactions. Commercial antibodies are typically raised against conserved epitopes in human DBNL, with validation across multiple species due to high sequence homology. Recent applications include exploring DBNL's role in viral entry mechanisms and synaptic plasticity, highlighting its multifaceted biological significance.
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