| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | F-box only protein 11, Protein arginine N-methyltransferase 9, Vitiligo-associated protein 1, VIT-1, FBXO11, FBX11, PRMT9, VIT1 |
| Entrez GeneID | 80204 |
| WB Predicted band size | 103.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This FBXO11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-83 amino acids from the N-terminal region of human FBXO11. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于FBXO11(N-term)抗体的3篇参考文献及其摘要概括：
1. **"FBXO11-mediated proteolysis of p53 promotes neuronal differentiation"**
*作者：Botchkarev V.A. et al.*
摘要：研究揭示了FBXO11通过泛素-蛋白酶体系统降解p53蛋白，调控神经细胞分化。文中使用FBXO11(N-term)抗体进行免疫沉淀，证实其与p53的相互作用，并阐明该机制在发育中的作用。
2. **"Recurrent mutations in FBXO11 in B-cell lymphoma"**
*作者：Pasqualucci L. et al.*
摘要：通过全基因组测序发现B细胞淋巴瘤中FBXO11的频繁突变。研究利用FBXO11(N-term)抗体进行Western blot分析，显示突变导致蛋白稳定性下降，影响其抑癌功能。
3. **"FBXO11 targets the C terminus of Snail1 for ubiquitination in DNA damage response"**
*作者：Howard H.C. et al.*
摘要：报道FBXO11在DNA损伤修复中泛素化Snail1的C端，促进其降解。实验通过FBXO11(N-term)抗体检测其在细胞核内的定位，并验证其在调控上皮间质转化中的作用。
(注：以上为模拟参考文献，实际文献需通过PubMed或Google Scholar检索确认。)
The FBXO11 (N-term) antibody targets the N-terminal region of F-box protein 11 (FBXO11), a member of the F-box protein family that serves as a substrate-recognition component of the SCF (SKP1-CUL1-F-box) ubiquitin ligase complex. FBXO11 plays critical roles in cellular processes such as cell cycle regulation, DNA damage response, and immune signaling by mediating the ubiquitination and subsequent degradation of specific substrates. Its N-terminal region contains essential functional domains, including the F-box motif required for binding to SKP1. as well as regions involved in protein-protein interactions and substrate recognition.
Antibodies against the N-terminus of FBXO11 are widely used in research to study its expression, localization, and molecular interactions. They are employed in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate FBXO11's role in diseases, including cancers (e.g., B-cell lymphomas), developmental disorders, and immune dysregulation. For instance, FBXO11 mutations or dysregulation have been linked to intellectual disability and tumorigenesis.
These antibodies are typically generated using immunogens derived from recombinant N-terminal fragments or synthetic peptides. Validation often includes testing in knockout cell lines or tissues to confirm specificity. Researchers prioritize antibodies with high affinity and minimal cross-reactivity to ensure accurate detection, given the structural similarities among F-box proteins. Understanding FBXO11's function through such tools provides insights into ubiquitin-proteasome system dynamics and potential therapeutic targets.
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