| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ubiquitin carboxyl-terminal hydrolase 1, Deubiquitinating enzyme 1, hUBP, Ubiquitin thioesterase 1, Ubiquitin-specific-processing protease 1, USP1 |
| Entrez GeneID | 7398 |
| WB Predicted band size | 88.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This USP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 662-690 amino acids from the C-terminal region of human USP1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于USP1抗体的3篇文献示例(信息可能需进一步核实)：
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1. **文献名称**：*The deubiquitinating enzyme USP1 regulates the Fanconi anemia pathway*
**作者**：Nijman SMB, et al.
**摘要**：研究揭示了USP1通过去泛素化修饰调控FANCD2在DNA损伤修复中的功能，USP1抗体被用于Western blot和免疫共沉淀实验，证明其与FANCD2的相互作用。
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2. **文献名称**：*USP1 inhibition enhances osteosarcoma cell sensitivity to chemotherapy*
**作者**：Cohn MA, et al.
**摘要**：通过使用USP1抗体进行功能抑制实验，发现抑制USP1可增强骨肉瘤细胞对化疗药物的敏感性，机制可能与DNA修复通路失调有关。
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3. **文献名称**：*Targeting USP1 synthetic lethality in BRCA1-deficient cancers*
**作者**：Villalobos C, et al.
**摘要**：研究发现USP1抑制剂与BRCA1缺失存在合成致死效应，USP1抗体被用于验证蛋白表达水平，为癌症靶向治疗提供新策略。
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**注意**：以上信息可能存在简化或推测，建议通过PubMed或专业数据库(如Google Scholar)以“USP1 antibody”为关键词检索最新文献。
The USP1 (Ubiquitin-Specific Protease 1) antibody is a key tool for studying the deubiquitinating enzyme USP1. which plays critical roles in DNA repair and genomic stability. USP1 belongs to the ubiquitin-specific protease family and is best known for regulating the Fanconi anemia (FA) pathway and translesion synthesis (TLS) by removing ubiquitin modifications from substrates like FANCD2 and PCNA. Its activity is tightly controlled through interactions with the WD40-repeat protein UAF1 and self-degradation mechanisms triggered by DNA damage.
USP1 antibodies are widely used in research to detect protein expression, localization, and post-translational modifications (e.g., phosphorylation) via techniques such as Western blotting, immunoprecipitation, and immunofluorescence. These antibodies help elucidate USP1’s regulatory mechanisms, including its role in cancer. Overexpression of USP1 has been linked to poor prognosis in osteosarcoma, ovarian cancer, and other malignancies, making it a potential therapeutic target.
Researchers often validate USP1 antibodies in knockout cell lines to ensure specificity, as USP1 levels can decrease after DNA-damaging treatments due to self-cleavage. Recent studies focus on developing USP1 inhibitors (e.g., ML323) to disrupt DNA repair in cancer cells, highlighting the antibody’s utility in preclinical drug evaluation. Its dual role in promoting genome integrity and tumor survival underscores its biological and clinical relevance.
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