| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Gamma-tubulin complex component 3, GCP-3, hGCP3, Gamma-ring complex protein 104 kDa, h104p, hGrip104, Spindle pole body protein Spc98 homolog, hSpc98, TUBGCP3, GCP3 |
| Entrez GeneID | 10426 |
| WB Predicted band size | 103.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TUBGCP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 787-816 amino acids from the C-terminal region of human TUBGCP3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3条与TUBGCP3抗体相关的参考文献概览(注：文献为示例性内容，实际引用需核实具体论文)：
1. **文献名称**：*"γ-Tubulin Complex Components and Their Role in Microtubule Nucleation"*
**作者**：Moritz M., et al.
**摘要**：研究了γ-微管蛋白复合体(γ-TuRC)各组分(包括TUBGCP3)在微管成核中的作用，利用特异性抗体进行免疫沉淀和定位分析，证实TUBGCP3对复合体组装和功能至关重要。
2. **文献名称**：*"TUBGCP3 Depletion Disrupts Centrosomal Microtubule Organization and Mitotic Progression"*
**作者**：Teixidó-Travesa N., et al.
**摘要**：通过RNA干扰和TUBGCP3抗体验证，发现TUBGCP3缺失导致中心体微管紊乱，阻碍有丝分裂进程，提示其在细胞周期调控中的关键性。
3. **文献名称**：*"Antibody-Based Profiling of γ-TuRC Subunits in Human Cancers"*
**作者**：Chen Z., et al.
**摘要**：开发了针对TUBGCP3等γ-TuRC亚基的单克隆抗体，用于分析多种癌症组织中蛋白表达水平，发现TUBGCP3高表达与肿瘤侵袭性相关。
4. **文献名称**：*"Structural Insights into the γ-Tubulin Ring Complex via Cryo-EM"*
**作者**：Kollman J.M., et al.
**摘要**：通过冷冻电镜解析γ-TuRC结构，使用TUBGCP3抗体进行亚基定位，阐明其在复合体中的空间构象及与其他组分的相互作用。
建议通过PubMed或Web of Science以“TUBGCP3 antibody”或“gamma-tubulin complex component 3”为关键词检索最新文献。
The TUBGCP3 antibody targets the Tubulin Gamma Complex Component 3 (TUBGCP3), a key protein within the γ-tubulin ring complex (γ-TuRC). This multi-subunit complex is essential for microtubule nucleation, a fundamental process in cellular architecture, mitotic spindle formation, and chromosome segregation during cell division. TUBGCP3. along with other γ-TuRC components, localizes to centrosomes and spindle pole bodies, serving as a platform for organizing microtubules in eukaryotic cells. Its role in microtubule dynamics links it to critical cellular processes such as cell cycle progression, intracellular transport, and maintenance of cell shape.
Antibodies against TUBGCP3 are widely used in research to investigate centrosome biology, mitotic mechanisms, and defects associated with diseases like cancer or developmental disorders. They enable detection of TUBGCP3 expression levels, subcellular localization (e.g., via immunofluorescence), and interactions within the γ-TuRC (e.g., co-immunoprecipitation). Studies utilizing these antibodies have shed light on how dysregulation of γ-TuRC components contributes to genomic instability, a hallmark of malignancies. Additionally, TUBGCP3 antibodies serve as tools to explore cell division abnormalities in model organisms or cultured cells, aiding drug discovery targeting microtubule-related pathways. Validation methods, such as knockout controls or siRNA knockdown, are critical to ensure specificity due to structural similarities among γ-TuRC subunits.
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