| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SURP and G-patch domain-containing protein 1, RNA-binding protein RBP, Splicing factor 4, SUGP1, SF4 |
| Entrez GeneID | 57794 |
| WB Predicted band size | 72.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-393 amino acids from the Central region of human SF4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 **SF4抗体** 的假设性参考文献示例(注：实际文献可能存在术语差异，建议结合具体领域核实)：
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1. **文献名称**：*SF4 Antibody Characterization in Spliceosome Complex Studies*
**作者**：Tanaka K. et al.
**摘要**：本文报道了一种高特异性SF4抗体的开发，用于研究其在剪接体复合物中的定位及功能。通过免疫沉淀和质谱分析，证实SF4与剪接因子相互作用，参与mRNA加工调控。
2. **文献名称**：*SF4 Autoantibodies as Biomarkers in Autoimmune Diseases*
**作者**：Rivera M. et al.
**摘要**：研究发现在部分系统性硬化症患者血清中存在SF4自身抗体，可能与其基因异常剪接相关。抗体检测为疾病诊断提供了潜在新靶点。
3. **文献名称**：*Role of SF4 in Embryonic Development: Insights from Antibody-Based Knockdown*
**作者**：Zhang Y. et al.
**摘要**：利用SF4抗体进行功能阻断实验，揭示SF4在小鼠胚胎神经管闭合中的关键作用，其缺失导致发育异常。
4. **文献名称**：*Commercial SF4 Antibody Validation for Cancer Research*
**作者**：Patel R. et al.
**摘要**：评估市售SF4抗体的特异性，发现其在乳腺癌组织中高表达，并与肿瘤侵袭性相关，提示SF4可能成为治疗靶点。
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**注意**：以上文献为示例性质，实际研究中需根据具体蛋白名称(如是否为SF3B4或其他剪接因子)检索权威数据库(如PubMed)。建议核实“SF4”术语准确性或提供更多背景信息以精准查询。
**Background of SF4 Antibody**
The SF4 antibody targets the splicing factor 3B subunit 4 (SF3B4), a critical component of the SF3B complex within the U2 small nuclear ribonucleoprotein (snRNP) particle. SF3B4 plays a pivotal role in pre-mRNA splicing, a process essential for gene expression regulation. It contributes to the recognition of branch site sequences during spliceosome assembly, ensuring accurate removal of introns and ligation of exons.
SF3B4 dysfunction has been linked to several diseases. Mutations in the *SF3B4* gene are associated with Nager syndrome, a rare congenital disorder characterized by craniofacial and limb abnormalities. Additionally, aberrant SF3B4 expression or activity is implicated in cancers, including leukemia and solid tumors, where altered splicing mechanisms promote oncogenesis or therapy resistance.
SF4 antibodies are widely used in research to study spliceosome dynamics, protein-RNA interactions, and disease mechanisms. They enable detection of SF3B4 via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Commercial SF4 antibodies are typically raised in rabbits or mice using recombinant protein fragments or synthetic peptides. Validation includes specificity checks using knockout cell lines or siRNA-mediated knockdown.
Understanding SF3B4's role through SF4 antibodies provides insights into splicing-related pathologies and potential therapeutic targets, highlighting its importance in both basic research and clinical contexts.
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