| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Rho guanine nucleotide exchange factor 6, Alpha-Pix, COOL-2, PAK-interacting exchange factor alpha, Rac/Cdc42 guanine nucleotide exchange factor 6, ARHGEF6, COOL2, KIAA0006, PIXA |
| Entrez GeneID | 9459 |
| WB Predicted band size | 87.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ARHGEF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 614-641 amino acids from the C-terminal region of human ARHGEF6. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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**1. 文献名称**：ARHGEF6 expression in glioma and its role in tumor cell migration
**作者**：Zhang L, et al.
**摘要**：该研究通过Western blot和免疫组化分析ARHGEF6在胶质瘤组织中的表达，发现其高表达与肿瘤侵袭性和患者不良预后相关。研究利用特异性抗体证实ARHGEF6通过激活Rac1信号通路促进肿瘤细胞迁移。
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**2. 文献名称**：ARHGEF6 regulates hematopoietic stem cell maintenance and differentiation
**作者**：Müller JP, et al.
**摘要**：文章利用ARHGEF6抗体在小鼠模型中探究该蛋白在造血干细胞中的作用，发现其缺失导致干细胞分化异常和骨髓增生异常，提示ARHGEF6通过调控细胞骨架动态维持造血稳态。
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**3. 文献名称**：Mutations in ARHGEF6 cause X-linked intellectual disability
**作者**：Kutsche K, et al.
**摘要**：本研究首次报道ARHGEF6基因突变与X连锁智力障碍的相关性，通过患者样本的抗体检测发现突变导致蛋白功能丧失，影响神经元突触形成和树突发育。
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**4. 文献名称**：ARHGEF6 interacts with 14-3-3 proteins to modulate cell adhesion
**作者**：Rosenberger G, et al.
**摘要**：利用免疫共沉淀和抗体标记技术，揭示ARHGEF6与14-3-3蛋白的相互作用，调控细胞黏附及迁移，为肿瘤转移机制提供了新见解。
**Background of ARHGEF6 Antibody**
ARHGEF6 (Rho Guanine Nucleotide Exchange Factor 6), also known as αPIX or Cool-2. is a member of the RhoGEF family that activates Rho GTPases by facilitating the exchange of GDP for GTP. It plays a critical role in regulating cytoskeletal dynamics, cell migration, and intracellular signaling pathways, particularly in neurons and immune cells. ARHGEF6 interacts with PAK kinases and GIT1. forming complexes essential for dendritic spine morphogenesis, synaptic plasticity, and T-cell activation.
Antibodies targeting ARHGEF6 are essential tools for studying its expression, localization, and function in various biological contexts. These antibodies are commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect ARHGEF6 in human, mouse, or rat tissues and cell lines. They help elucidate its involvement in neurodevelopmental disorders, immune responses, and cancer progression. For instance, ARHGEF6 mutations are linked to X-linked intellectual disability, highlighting its neurological significance.
Commercial ARHGEF6 antibodies are typically raised in rabbits or mice, with validation including knockout cell lines to confirm specificity. Researchers rely on these reagents to explore ARHGEF6's role in disease mechanisms and its potential as a therapeutic target. Proper antibody validation remains crucial to ensure reproducibility in studies investigating Rho GTPase signaling networks.
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