| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PR domain zinc finger protein 8, 211-, PR domain-containing protein 8, PRDM8, PFM5 |
| Entrez GeneID | 56978 |
| WB Predicted band size | 71.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PRDM8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-102 amino acids from the N-terminal region of human PRDM8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及PRDM8(N-term)抗体的参考文献及其摘要内容概括：
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1. **文献名称**: *PRDM8 regulates transcriptional repression during postnatal neuronal development*
**作者**: Yang X, et al.
**摘要**: 本研究利用针对PRDM8 N端的特异性抗体，在小鼠脑组织中发现PRDM8通过其N端结构域招募组蛋白甲基转移酶复合物，调控神经元成熟过程中的基因沉默，并证实其在突触可塑性中的作用。
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2. **文献名称**: *Epigenetic control of cerebellar morphogenesis by PRDM8*
**作者**: Garcia-Garcia A, et al.
**摘要**: 通过PRDM8(N-term)抗体的免疫组化分析，揭示了PRDM8在小脑发育中的时空表达模式，并证明其N端介导的组蛋白修饰活性对颗粒神经元迁移和分化的关键调控作用。
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3. **文献名称**: *PRDM8 interacts with CUL3–SPOP ubiquitin ligase to suppress medulloblastoma progression*
**作者**: Li H, et al.
**摘要**: 研究使用PRDM8 N端特异性抗体进行Co-IP实验，发现PRDM8通过N端与SPOP蛋白结合，促进致癌蛋白降解，抑制髓母细胞瘤生长，为靶向治疗提供了新机制。
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注：上述文献为示例性描述，实际文献需根据具体数据库(如PubMed、Google Scholar)检索确认。部分研究可能需结合抗体验证数据(如Western blot结果)进一步筛选。
The PRDM8 (N-term) antibody is a tool designed to detect the N-terminal region of PRDM8 (PR/SET Domain 8), a member of the PRDI-BF1-RIZ homology (PR) domain-containing protein family. PRDM8 functions as a transcriptional regulator, primarily through its N-terminal PR domain, which exhibits histone methyltransferase activity, and its C-terminal zinc finger motifs for DNA binding. It plays critical roles in neuronal development, synaptic connectivity, and the maintenance of inhibitory neuronal circuits. Studies highlight its importance in cortical and cerebellar development, where it suppresses premature differentiation of neural progenitor cells and regulates excitatory-inhibitory balance in the brain.
The N-terminal-specific antibody enables researchers to study PRDM8's expression, localization, and functional interactions in tissues and cells. It is widely used in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to investigate PRDM8's role in neurodevelopmental disorders, epilepsy, and cancer, where dysregulation of PRDM8 has been implicated. For instance, PRDM8 downregulation is associated with poor prognosis in certain tumors, while mutations are linked to neurological deficits in mouse models. The antibody's specificity for the N-terminal region ensures detection of full-length PRDM8. avoiding cross-reactivity with truncated isoforms or homologous PR family members. Validation typically includes testing in PRDM8-knockout models or siRNA-treated cells to confirm target specificity.
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