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Rabbit Polyclonal MED16 Antibody

  • 中文名: MED16抗体
  • 别    名: Mediator of RNA polymerase II transcription subunit 16, Mediator complex subunit 16, Thyroid hormone receptor-associated protein 5, Thyroid hormone receptor-associated protein complex 95 kDa component, Trap95, Vitamin D3 receptor-interacting protein compl
货号: IPDX31678
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesMediator of RNA polymerase II transcription subunit 16, Mediator complex subunit 16, Thyroid hormone receptor-associated protein 5, Thyroid hormone receptor-associated protein complex 95 kDa component, Trap95, Vitamin D3 receptor-interacting protein complex 92 kDa component, DRIP92, MED16, DRIP92 {ECO:0000312|EMBL:AAD310871}, THRAP5
Entrez GeneID10025
WB Predicted band size96.8kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis MED16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-281 amino acids from the Central region of human MED16.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于MED16抗体的参考文献示例(注:以下内容为虚构,仅用于演示格式):

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1. **文献名称**: "MED16 regulates oxidative stress response via transcriptional control in yeast"

**作者**: Smith A, et al.

**摘要**: 本研究利用MED16特异性抗体进行染色质免疫沉淀(ChIP)和Western blot分析,揭示了MED16在中介体复合物中调控氧化应激相关基因的作用机制,证实其通过结合特定启动子区域激活应激应答通路。

2. **文献名称**: "Developmental role of MED16 in Drosophila neural differentiation"

**作者**: Zhang L, et al.

**摘要**: 通过免疫荧光和免疫印迹技术(使用MED16抗体),作者发现MED16在果蝇神经发育中调控关键分化基因的表达,缺失导致神经组织发育异常,表明其在胚胎发育中的必要性。

3. **文献名称**: "MED16 interacts with p53 to modulate tumor suppressor pathways"

**作者**: Lee H, et al.

**摘要**: 该研究采用MED16抗体进行共免疫沉淀和免疫组化实验,证明MED16与p53直接相互作用,影响其靶基因的转录活性,并在结直肠癌样本中观察到MED16表达下调。

4. **文献名称**: "Subcellular localization of Mediator complex subunit MED16 under heat shock"

**作者**: Johnson R, et al.

**摘要**: 利用MED16抗体的免疫细胞化学分析,发现热休克条件下MED16从细胞核向应激颗粒的动态转位,提示其可能参与应激相关的转录暂停调控。

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(注:以上文献为示例性内容,实际引用时请核实真实文献。)

背景信息

The MED16 antibody is a research tool designed to target the MED16 protein, a subunit of the Mediator complex—a multi-protein assembly critical for regulating RNA polymerase II (Pol II)-mediated transcription in eukaryotes. The Mediator complex acts as a molecular bridge, facilitating communication between transcription factors and the basal transcriptional machinery. MED16. also known as Thyroid Hormone Receptor-Associated Protein 28 (TRAP28), plays a role in stabilizing the Mediator structure and modulating its interactions with specific transcription factors. It is implicated in diverse cellular processes, including stress responses, lipid metabolism, and developmental signaling pathways. Dysregulation of MED16 has been linked to diseases such as cancer and metabolic disorders, underscoring its biological relevance. Antibodies against MED16 are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to study its expression, localization, and protein-protein interactions. These tools help elucidate MED16's functional mechanisms, its contribution to Mediator-dependent transcriptional regulation, and its potential role in disease pathogenesis. Researchers also employ MED16 antibodies to explore tissue-specific expression patterns and post-translational modifications, providing insights into its regulatory versatility in gene expression networks.

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