WB | 1/1000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | Mediator of RNA polymerase II transcription subunit 16, Mediator complex subunit 16, Thyroid hormone receptor-associated protein 5, Thyroid hormone receptor-associated protein complex 95 kDa component, Trap95, Vitamin D3 receptor-interacting protein complex 92 kDa component, DRIP92, MED16, DRIP92 {ECO:0000312|EMBL:AAD310871}, THRAP5 |
Entrez GeneID | 10025 |
WB Predicted band size | 96.8kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This MED16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-281 amino acids from the Central region of human MED16. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MED16抗体的参考文献示例(注:以下内容为虚构,仅用于演示格式):
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1. **文献名称**: "MED16 regulates oxidative stress response via transcriptional control in yeast"
**作者**: Smith A, et al.
**摘要**: 本研究利用MED16特异性抗体进行染色质免疫沉淀(ChIP)和Western blot分析,揭示了MED16在中介体复合物中调控氧化应激相关基因的作用机制,证实其通过结合特定启动子区域激活应激应答通路。
2. **文献名称**: "Developmental role of MED16 in Drosophila neural differentiation"
**作者**: Zhang L, et al.
**摘要**: 通过免疫荧光和免疫印迹技术(使用MED16抗体),作者发现MED16在果蝇神经发育中调控关键分化基因的表达,缺失导致神经组织发育异常,表明其在胚胎发育中的必要性。
3. **文献名称**: "MED16 interacts with p53 to modulate tumor suppressor pathways"
**作者**: Lee H, et al.
**摘要**: 该研究采用MED16抗体进行共免疫沉淀和免疫组化实验,证明MED16与p53直接相互作用,影响其靶基因的转录活性,并在结直肠癌样本中观察到MED16表达下调。
4. **文献名称**: "Subcellular localization of Mediator complex subunit MED16 under heat shock"
**作者**: Johnson R, et al.
**摘要**: 利用MED16抗体的免疫细胞化学分析,发现热休克条件下MED16从细胞核向应激颗粒的动态转位,提示其可能参与应激相关的转录暂停调控。
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(注:以上文献为示例性内容,实际引用时请核实真实文献。)
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