| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Tight junction protein ZO-3, Tight junction protein 3, Zona occludens protein 3, Zonula occludens protein 3, TJP3, ZO3 |
| Entrez GeneID | 27134 |
| WB Predicted band size | 101.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TJP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-129 amino acids from the N-terminal region of human TJP3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于TJP3(N-term)抗体的模拟参考文献示例，供参考：
---
1. **文献名称**：*TJP3 regulates epithelial barrier function through N-terminal domain interactions*
**作者**：Smith A.B., et al.
**摘要**：本研究探讨了TJP3蛋白N端结构域在上皮细胞紧密连接中的功能。通过使用TJP3(N-term)抗体进行免疫荧光和免疫共沉淀实验，发现TJP3与occludin蛋白直接互作，调控肠上皮屏障通透性。研究为炎症性肠病提供了潜在机制。
2. **文献名称**：*Expression profiling of TJP3 in hepatocellular carcinoma using a novel N-terminal specific antibody*
**作者**：Chen L., Wang X., et al.
**摘要**：利用TJP3(N-term)抗体对肝癌组织进行Western blot和免疫组化分析，发现TJP3在癌旁组织中高表达，且与患者预后相关。结果表明，TJP3可能通过维持细胞极性抑制肿瘤转移。
3. **文献名称**：*Phosphorylation dynamics of TJP3 N-terminal domain during cell migration*
**作者**：Rodriguez M., et al.
**摘要**：研究聚焦于TJP3 N端结构域的磷酸化修饰在细胞迁移中的作用。通过TJP3(N-term)抗体检测发现，EGF信号可诱导该区域磷酸化，破坏其与ZO-1的结合，促进上皮-间质转化(EMT)。
4. **文献名称**：*TJP3 knockdown alters synaptic stability in neuronal cells: Evidence from N-terminal antibody-based assays*
**作者**：Kumar S., Lee J.H.
**摘要**：通过siRNA敲低TJP3并结合N-term抗体的免疫印迹分析，揭示TJP3在神经元突触稳定性中的作用。结果显示，TJP3缺失导致突触蛋白错误定位，影响神经传递效率。
---
**注**：以上文献为模拟示例，实际引用需查询PubMed、Google Scholar等数据库获取真实信息。若需具体文献，建议通过关键词“TJP3 antibody N-terminal”或抗体货号(如Santa Cruz Biotechnology的sc-517429)检索。
TJP3 (Tight Junction Protein 3), also known as ZO-3. is a member of the zonula occludens (ZO) family of scaffolding proteins that play critical roles in maintaining cell polarity and barrier function in epithelial and endothelial tissues. It localizes to tight junctions, specialized membrane domains that regulate paracellular transport and cell-cell adhesion. TJP3 interacts with other junctional proteins, including claudins, occludin, and the cytoskeleton, to stabilize junctional complexes and mediate signaling pathways.
The TJP3 (N-term) antibody is a commonly used tool targeting the N-terminal region of the TJP3 protein. This region contains PDZ domains essential for protein-protein interactions, enabling TJP3 to anchor transmembrane proteins to the actin cytoskeleton. Researchers employ this antibody in techniques like Western blotting (WB), immunofluorescence (IF), and immunohistochemistry (IHC) to study TJP3 expression, localization, and its dynamic changes under physiological or pathological conditions, such as epithelial barrier dysfunction, inflammation, or cancer metastasis. The N-terminal specificity helps distinguish TJP3 from homologous ZO proteins (ZO-1. ZO-2) and may assist in detecting isoforms or truncation variants. Validation typically includes testing in knockout models or siRNA-treated cells to confirm signal specificity.
×
