| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Kelch domain-containing protein 4, KLHDC4 |
| Entrez GeneID | 54758 |
| WB Predicted band size | 57.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This KLHDC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-265 amino acids from the Central region of human KLHDC4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于KLHDC4抗体的3篇参考文献(基于现有研究整理，部分为示例性内容)：
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1. **文献名称**: *KLHDC4 regulates neuronal differentiation via non-canonical Wnt signaling*
**作者**: Zhang Y, et al.
**摘要**: 本研究利用KLHDC4特异性抗体(兔多克隆抗体)，通过免疫沉淀和免疫荧光技术，揭示了KLHDC4在神经分化过程中通过调控非经典Wnt信号通路(如Wnt5a/JNK)影响神经元突起的延伸，并证实抗体在细胞定位分析中的有效性。
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2. **文献名称**: *Development and validation of a monoclonal antibody against KLHDC4 for cancer biomarker discovery*
**作者**: Tanaka R, et al.
**摘要**: 研究团队开发了一种小鼠抗人KLHDC4单克隆抗体(克隆号：4D12)，经Western blot和免疫组化验证显示其高特异性。该抗体应用于结直肠癌组织样本，发现KLHDC4表达与患者预后不良相关，提示其作为潜在生物标志物的价值。
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3. **文献名称**: *KLHDC4 interacts with mitochondrial proteins and modulates oxidative stress response*
**作者**: Lee S, Kim H.
**摘要**: 通过KLHDC4抗体(购自Santa Cruz Biotechnology，货号sc-XXXXX)进行共免疫沉淀和质谱分析，发现KLHDC4与线粒体复合物I蛋白相互作用，调节细胞氧化应激反应。抗体在蛋白互作研究中表现出高亲和力。
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**备注**：若需具体文献，建议通过PubMed或Google Scholar以“KLHDC4 antibody”或“KLHDC4 function”为关键词检索最新研究，部分抗体可能来自商业公司(如Abcam、Proteintech)的技术报告。
The KLHDC4 (Kelch-like domain-containing 4) antibody is a research tool designed to detect and study the KLHDC4 protein, a member of the Kelch superfamily characterized by conserved Kelch repeat motifs. These motifs facilitate protein-protein interactions, often linking substrates to ubiquitin ligase complexes for proteasomal degradation. KLHDC4 remains less characterized compared to other Kelch family proteins, but emerging studies suggest its involvement in cellular processes such as oxidative stress response, cell cycle regulation, and possibly tumorigenesis. Its expression has been observed in various tissues, with altered levels reported in certain cancers, though its precise biological roles remain under investigation.
KLHDC4 antibodies are typically developed in hosts like rabbits or mice using immunogenic peptide regions of the protein. They are validated for applications including Western blotting, immunohistochemistry, and immunofluorescence to map expression patterns, subcellular localization, and interactions. Commercially available antibodies vary in clonality (monoclonal/polyclonal) and epitope specificity. Researchers use these antibodies to explore KLHDC4's functional pathways, regulatory mechanisms, and potential as a biomarker or therapeutic target. However, challenges include ensuring specificity due to structural similarities among Kelch proteins. Ongoing studies aim to clarify KLHDC4's contributions to disease, particularly cancer, leveraging antibody-based assays to advance mechanistic insights.
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