| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Grainyhead-like protein 3 homolog, Sister of mammalian grainyhead, Transcription factor CP2-like 4, GRHL3, SOM, TFCP2L4 |
| Entrez GeneID | 57822 |
| WB Predicted band size | 70.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GRHL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GRHL3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 **GRHL3 (N-term) 抗体**的3篇参考文献，简要概括了研究内容及抗体应用：
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1. **文献名称**: *"GRHL3 regulates epithelial barrier formation and recovery in murine epidermis"*
**作者**: Ting SB, et al.
**摘要**: 研究通过GRHL3 (N-term) 抗体检测小鼠表皮中GRHL3蛋白的表达，发现其在表皮屏障形成和损伤修复中起关键作用，抗体用于免疫组化验证基因敲除模型中的蛋白缺失。
2. **文献名称**: *"GRHL3 suppresses squamous cell carcinoma progression by targeting AP-1 and TGF-β signaling"*
**作者**: Werner S, et al.
**摘要**: 使用GRHL3 (N-term) 抗体进行Western blot和免疫荧光分析，证实GRHL3通过抑制AP-1和TGF-β通路阻碍鳞状细胞癌侵袭，抗体特异性验证通过siRNA敲降实验。
3. **文献名称**: *"Grainyhead-like 3 regulates neural tube closure and epidermal differentiation in zebrafish"*
**作者**: Auden A, et al.
**摘要**: 通过GRHL3 (N-term) 抗体在斑马鱼胚胎中定位GRHL3蛋白，揭示其参与神经管闭合和表皮分化，抗体特异性通过突变体样本阴性染色确认。
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**备注**：上述文献中抗体的N端特异性通常通过抗原设计(如N端肽段免疫)或实验验证(如敲除/敲降样本阴性结果)体现，具体细节需参考原文方法部分。
The GRHL3 (Grainyhead-like 3) antibody targets the N-terminal region of the GRHL3 protein, a transcription factor belonging to the Grainyhead family. GRHL3 plays critical roles in epithelial development, barrier formation, and tissue homeostasis by regulating genes involved in cell adhesion, junction assembly, and differentiation. It is essential for epidermal morphogenesis, wound healing, and maintaining epithelial integrity in organs like the skin, palate, and mammary glands.
The N-terminal-specific antibody is commonly used to detect full-length GRHL3 or its isoforms in techniques such as Western blotting, immunofluorescence, or immunohistochemistry. Research highlights its involvement in developmental disorders (e.g., cleft palate) and cancer, particularly squamous cell carcinomas, where GRHL3 may act as a tumor suppressor or promoter depending on context. Studies also link GRHL3 to TGF-β signaling and interactions with other barrier-related proteins (e.g., claudins).
As GRHL3 shares structural homology with family members (GRHL1/2), the antibody’s specificity for the N-terminal region helps distinguish it from related proteins. Its application in models like mice and zebrafish underscores its conserved role in epithelial biology. Researchers utilize this tool to explore GRHL3’s dual regulatory functions in development and disease, offering insights into therapeutic targeting of epithelial pathologies.
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