| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Beta-1,3-N-acetylglucosaminyltransferase radical fringe, O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase, RFNG |
| Entrez GeneID | 5986 |
| WB Predicted band size | 36.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RFNG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 282-310 amino acids from the C-terminal region of human RFNG. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于RFNG抗体的模拟参考文献示例(注：文献为虚构，仅供格式参考，实际需查询数据库获取)：
---
1. **文献名称**: *Antibody-based targeting of RFNG disrupts Notch signaling in colorectal cancer*
**作者**: Lee, J., et al.
**摘要**: 研究利用特异性RFNG单克隆抗体抑制结直肠癌细胞中的Notch通路，证实RFNG通过调控Jagged1-Notch1相互作用促进肿瘤侵袭，抗体干预可降低转移潜能。
2. **文献名称**: *RFNG antibody characterization in murine limb development*
**作者**: Garcia, M., et al.
**摘要**: 通过免疫组化和Western blot验证新型多克隆RFNG抗体的特异性，揭示其在小鼠肢体发育过程中调控细胞边界形成的时空表达模式。
3. **文献名称**: *High-throughput screening identifies RFNG as a therapeutic target in glioblastoma using functional antibodies*
**作者**: Wang, Y., et al.
**摘要**: 采用高通量抗体筛选平台，发现靶向RFNG的抗体可抑制胶质母细胞瘤细胞增殖，机制涉及Notch通路下游靶点Hes1的下调。
4. **文献名称**: *Cross-reactivity analysis of anti-RFNG antibodies across species for translational research*
**作者**: Tanaka, K., et al.
**摘要**: 评估多种商业RFNG抗体的交叉反应性，确定某克隆号抗体在人类、小鼠及斑马鱼模型中的一致性应用，支持跨物种发育机制研究。
---
**提示**：实际文献需通过PubMed、Web of Science等平台检索，建议使用关键词“RFNG antibody”或“Radial fringe + antibody”并结合时间过滤器(如2018-2023)获取最新研究。
RFNG (Radial Fringe Glycosyltransferase) antibodies are tools used to study the RFNG protein, a key enzyme in the Notch signaling pathway. RFNG, a member of the fringe family (Lunatic, Manic, and Radical fringe), modulates Notch receptor activity by adding O-fucose glycans to epidermal growth factor (EGF)-like repeats in the Notch extracellular domain. This post-translational modification fine-tunes cell-cell communication critical for embryonic development, tissue patterning, and stem cell maintenance.
Discovered in the late 1990s, RFNG gained attention for its role in somite segmentation and neural development. Dysregulation of RFNG-mediated Notch signaling is linked to congenital disorders and cancers, particularly T-cell acute lymphoblastic leukemia. RFNG antibodies enable researchers to detect protein expression, localization, and interaction dynamics in developmental models and disease contexts. They are widely used in immunohistochemistry, Western blotting, and functional studies to dissect RFNG's regulatory mechanisms. Recent studies also explore RFNG's involvement in angiogenesis and metabolic diseases, highlighting its multifaceted roles. These antibodies remain essential for unraveling Notch pathway complexity and developing targeted therapies for RFNG-associated pathologies.
×
