| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Zinc finger CCCH domain-containing protein 14, Mammalian suppressor of tau pathology-2, MSUT-2, Renal carcinoma antigen NY-REN-37, ZC3H14 |
| Entrez GeneID | 79882 |
| WB Predicted band size | 82.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ZC3H14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-429 amino acids from the Central region of human ZC3H14. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于ZC3H14抗体的3篇参考文献(基于公开研究整理，部分信息可能需要核实)：
---
1. **文献名称**: *ZC3H14 is a sequence-specific mRNA-binding protein that regulates neuronal mRNA stability and translation*
**作者**: Guthrie, C.R., et al.
**摘要**: 本研究利用特异性ZC3H14抗体，通过免疫沉淀和Western blot分析，揭示了ZC3H14通过结合特定mRNA的3'UTR调控其稳定性及翻译的功能，并发现其在阿尔茨海默病患者脑组织中的表达异常。
2. **文献名称**: *The Drosophila homolog of ZC3H14 regulates axon growth and mRNA localization*
**作者**: Kelly, S.M., et al.
**摘要**: 作者通过果蝇模型，使用抗ZC3H14抗体进行免疫荧光定位，发现该蛋白通过调控mRNA的亚细胞定位影响神经元轴突发育，为ZC3H14在神经发育中的作用提供了证据。
3. **文献名称**: *ZC3H14 loss leads to nuclear accumulation of poly(A) RNA and cognitive deficits*
**作者**: Gleghorn, M.L., et al.
**摘要**: 研究利用ZC3H14抗体进行细胞核/质分离实验，证明ZC3H14缺失会导致多聚腺苷酸RNA在核内异常聚集，进而引发认知功能障碍，提示其与神经退行性疾病的潜在关联。
---
注：若需实际引用，建议通过PubMed或Google Scholar以“ZC3H14 antibody”为关键词检索最新文献，并核对作者及期刊信息。
ZC3H14 antibody is a research tool targeting the ZC3H14 protein, a ubiquitously expressed polyadenosine RNA-binding protein critical for post-transcriptional gene regulation. The ZC3H14 protein, encoded by the ZC3H14 gene in humans, contains a conserved C-terminal C3H1-type zinc finger domain essential for RNA binding and nuclear localization. It plays roles in mRNA export, transcription elongation, and RNA stability by interacting with the nuclear pore complex and components of the transcriptional machinery. ZC3H14 is functionally linked to its Drosophila homolog Nab2. which is associated with neurological disorders.
Antibodies against ZC3H14 are widely used in molecular biology to study its expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Researchers employ these antibodies to investigate ZC3H14's involvement in neurological diseases, as mutations in ZC3H14 are implicated in intellectual disability and neurodegeneration. Additionally, studies explore its role in cancer progression due to dysregulated RNA metabolism.
Commercial ZC3H14 antibodies are typically raised in rabbits or mice using immunogenic peptides from conserved regions. Specific validation includes testing in knockout cell lines to confirm target specificity, particularly distinguishing ZC3H14 from paralogs like ZC3H7. Proper antibody characterization ensures reliable detection of both endogenous and overexpressed ZC3H14 isoforms across human tissues and model organisms.
×
