| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Thyroid hormone receptor-associated protein 3, Thyroid hormone receptor-associated protein complex 150 kDa component, Trap150, THRAP3, TRAP150 |
| Entrez GeneID | 9967 |
| WB Predicted band size | 108.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This THRAP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 927-955 amino acids from the C-terminal region of human THRAP3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于THRAP3抗体的3篇参考文献示例(注：文献为虚拟示例，实际文献需根据具体研究补充)：
1. **"THRAP3 regulates the RNA-binding activity of TLS in transcriptional repression"**
- 作者：Smith A, et al.
- 摘要：研究通过THRAP3抗体进行免疫共沉淀实验，揭示THRAP3与RNA结合蛋白TLS相互作用，调控DNA损伤应答中的转录抑制功能。
2. **"THRAP3 modulates alternative splicing by bridging spliceosome components"**
- 作者：Chen L, et al.
- 摘要：利用THRAP3抗体的免疫荧光技术，发现THRAP3作为剪接体中介因子，通过连接U1 snRNP和剪接因子调控选择性剪接过程。
3. **"THRAP3 promotes tumorigenesis by stabilizing MYC mRNA in colorectal cancer"**
- 作者：Wang Y, et al.
- 摘要：通过Western blot和RNA免疫沉淀(RIP)结合THRAP3抗体，证明THRAP3通过结合MYC mRNA的3'UTR增强其稳定性，促进结直肠癌进展。
如需具体文献，建议在PubMed或Web of Science中检索关键词“THRAP3 antibody”或“THRAP3 function”。
THRAP3 (Thyroid Hormone Receptor Associated Protein 3), also known as TRAP150 or BCLAF1 (Bcl-2-associated transcription factor 1), is a multifunctional protein implicated in transcriptional regulation, pre-mRNA splicing, and cellular stress responses. It belongs to the TRAP complex, which interacts with nuclear hormone receptors, including thyroid hormone receptors, to modulate gene expression. THRAP3 contains domains that facilitate RNA binding and protein-protein interactions, enabling its role in bridging transcription and splicing machinery. Studies suggest its involvement in cell cycle regulation, apoptosis, and DNA damage repair, with dysregulation linked to cancers, neurological disorders, and viral infections.
Antibodies targeting THRAP3 are essential tools for investigating its molecular functions and localization. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect endogenous THRAP3 in various cell lines and tissues. Commercially available THRAP3 antibodies are typically raised against specific epitopes, such as the N-terminal or C-terminal regions, and validated for specificity across human, mouse, and rat models. Researchers employ these antibodies to study THRAP3’s interaction networks, spliceosome dynamics, and roles in diseases. For instance, elevated THRAP3 levels have been observed in certain cancers, correlating with tumor progression and therapy resistance. However, challenges remain in distinguishing THRAP3 isoforms due to alternative splicing, necessitating careful antibody validation. Overall, THRAP3 antibodies are pivotal in unraveling its dual roles in gene expression and cellular homeostasis.
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