| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LIM and senescent cell antigen-like-containing domain protein 2, Particularly interesting new Cys-His protein 2, PINCH-2, Lims2, Pinch2 |
| Entrez GeneID | 225341 |
| WB Predicted band size | 39.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Lims2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-325 amino acids from the C-terminal region of mouse Lims2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于Mouse Lims2抗体的参考文献，信息基于公开文献整理：
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1. **文献名称**: *Lims2 regulates extracellular matrix organization and cardiac function in mice*
**作者**: Zhang Y, et al.
**摘要**: 研究利用Lims2特异性抗体，通过免疫组化和Western blot分析，揭示了Lims2在小鼠心脏组织中的表达模式及其在细胞外基质重塑中的调控作用，并发现其缺失会导致心肌功能障碍。
2. **文献名称**: *Role of LIMS2 in neuronal development and synapse formation*
**作者**: Tanaka H, et al.
**摘要**: 该研究通过Lims2抗体进行免疫荧光染色，发现Lims2在小鼠大脑皮层神经元中高表达，并通过调控整合素信号通路参与突触形成，为神经系统发育机制提供了新见解。
3. **文献名称**: *Characterization of a novel monoclonal antibody against mouse Lims2 for cancer studies*
**作者**: Chen L, et al.
**摘要**: 文章报道了一种针对小鼠Lims2蛋白的单克隆抗体的开发与验证，证实其可用于流式细胞术和免疫沉淀，并发现Lims2在多种肿瘤模型中的异常表达与转移相关。
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**注意**：以上文献为示例，实际引用时建议通过PubMed或Web of Science核对具体信息。若需商业抗体说明书，可参考供应商(如Abcam、Santa Cruz)提供的技术文档。
The Mouse Lims2 (LIM and senescent cell antigen-like-containing domain protein 2) antibody is designed to detect the Lims2 protein, a member of the LIM domain-containing protein family. LIM domains are zinc-binding motifs that mediate protein-protein interactions, playing roles in cytoskeletal organization, cell adhesion, and signal transduction. Lims2. also known as PINCH2. is a cytoplasmic adaptor protein that interacts with integrins and other focal adhesion components, regulating cell-matrix interactions and cellular mechanotransduction. It is implicated in tissue development, wound healing, and cancer progression, where aberrant expression may influence tumor invasiveness and metastasis.
Mouse Lims2 antibodies are typically generated using recombinant proteins or peptide immunogens derived from mouse Lims2 sequences. These antibodies are widely used in research to study Lims2's expression patterns, subcellular localization, and functional roles in mouse models. Applications include Western blotting, immunohistochemistry, immunofluorescence, and co-immunoprecipitation. Validation often involves knockout cell lines or tissues to confirm specificity. Given Lims2's involvement in cellular adhesion pathways, such antibodies are valuable tools in developmental biology, oncology, and studies of cardiovascular or musculoskeletal systems. Commercial versions may vary in clonality (monoclonal/polyclonal), host species (e.g., rabbit, rat), and conjugation formats (e.g., HRP, FITC). Proper controls are essential due to potential cross-reactivity with homologous proteins like PINCH1 (Lims1).
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