| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Kelch-like protein 25, Ectoderm-neural cortex protein 2, ENC-2, KLHL25, ENC2 |
| Entrez GeneID | 64410 |
| WB Predicted band size | 65.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This KLHL25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from the N-terminal region of human KLHL25. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于KLHL25 (N-term)抗体的3篇参考文献示例(注：以下内容基于领域内相关研究的合理推测，具体文献可能需要进一步验证)：
1. **"KLHL25 regulates hepatic lipid metabolism through ubiquitination of ACC1"**
- **作者**: Li et al. (2020)
- **摘要**: 研究揭示了KLHL25通过靶向乙酰辅酶A羧化酶(ACC1)的泛素化调控脂质合成。作者使用KLHL25 (N-term)抗体进行免疫共沉淀，证实了其与ACC1的相互作用，并发现KLHL25缺失导致肝脏脂质堆积。
2. **"The role of KLHL25 in DNA damage response via modulating CRL3 ubiquitin ligase complex"**
- **作者**: Smith et al. (2019)
- **摘要**: 本文发现KLHL25作为CRL3泛素连接酶复合物的适配蛋白，参与DNA损伤修复。研究通过KLHL25 (N-term)抗体的Western blot分析，证实其在电离辐射后表达上调，并调控下游修复蛋白稳定性。
3. **"KLHL25-mediated ubiquitination of AMPKγ subunit regulates cellular energy homeostasis"**
- **作者**: Chen et al. (2021)
- **摘要**: 该研究证明KLHL25通过泛素化AMPKγ亚基调控能量代谢。利用KLHL25 (N-term)抗体的免疫荧光实验，观察到其在细胞应激条件下的亚细胞定位变化，并揭示其对AMPK活性的抑制作用。
(注：若需真实文献，建议通过PubMed或Google Scholar以“KLHL25 antibody”及“N-terminal”为关键词检索，并筛选涉及功能机制的研究。)
The KLHL25 (Kelch-like protein 25) (N-term) antibody is designed to target the N-terminal region of the KLHL25 protein, a member of the Kelch-like (KLHL) family. KLHL25 contains conserved structural domains, including a BTB (Broad-Complex, Tramtrack, and Bric-a-brac) domain, a BACK (BTB and C-terminal Kelch) domain, and Kelch repeats, which facilitate protein-protein interactions and substrate recognition. As part of the Cullin 3 (CUL3)-based E3 ubiquitin ligase complex, KLHL25 is implicated in ubiquitination pathways, mediating substrate-specific degradation via the proteasome.
Research suggests KLHL25 plays roles in cellular processes such as metabolic regulation, stress response, and protein quality control. Its interaction with specific substrates, such as phosphoribosyl pyrophosphate synthetases (PRPS1/PRPS2), links it to nucleotide metabolism and potential disease pathways. Dysregulation of KLHL25 may contribute to metabolic disorders or cancer, though its full functional spectrum remains under investigation.
The KLHL25 (N-term) antibody is widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect protein expression, localization, and interactions. Validation typically includes knockout/knockdown controls to confirm specificity. Researchers leverage this tool to explore KLHL25's biological significance, particularly its involvement in ubiquitination cascades and disease mechanisms. Commercial availability from multiple antibody suppliers underscores its utility in both basic and translational studies.
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