| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Polycystic kidney disease 2-like 1 protein, Polycystin-2 homolog, Polycystin-2L1, Polycystin-L, Polycystin-L1, PKD2L1, PKD2L, PKDL, TRPP3 |
| Entrez GeneID | 9033 |
| WB Predicted band size | 92.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PKD2L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-65 amino acids from the N-terminal region of human PKD2L1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PKD2L1 (N-term)抗体的参考文献概览：
1. **"PKD2L1 is a cation channel involved in sour taste transduction"**
- **作者**: Ishimaru Y. et al.
- **摘要**: 该研究使用PKD2L1 (N-term)抗体定位味觉细胞中的PKD2L1蛋白，发现其在酸味感知中作为阳离子通道的关键作用。通过免疫组化证实其在特定味蕾细胞中的表达。
2. **"Cerebrospinal fluid pH regulation by PKD2L1-expressing neurons"**
- **作者**: Huang AL. et al.
- **摘要**: 利用N端特异性抗体进行小鼠脑脊液pH值调控机制研究，证明表达PKD2L1的神经元通过检测pH变化参与中枢神经系统的酸碱平衡调节。
3. **"PKD2L1 in renal tubular acid-base sensing"**
- **作者**: Delling M. et al.
- **摘要**: 通过Western blot和免疫荧光技术，结合PKD2L1 (N-term)抗体，揭示该蛋白在肾小管上皮细胞中的表达及对局部酸碱环境的响应功能，提示其与肾脏疾病潜在关联。
4. **"Characterization of polycystin-2 family proteins in cilia signaling"**
- **作者**: Yoder BK. et al.
- **摘要**: 研究多囊蛋白家族成员在纤毛信号中的作用，使用N端抗体验证PKD2L1在纤毛膜上的定位，并探讨其与PKD1/PKD2的功能差异及疾病病理关系。
这些文献均通过PKD2L1的N端抗体进行蛋白定位或功能验证，涵盖味觉、神经系统、肾脏生理及纤毛生物学等领域。
The PKD2L1 (N-term) antibody is designed to target the N-terminal region of the polycystic kidney disease 2-like 1 protein (PKD2L1), a non-selective cation channel belonging to the transient receptor potential polycystin (TRPP) family. PKD2L1 is implicated in various physiological processes, including acid sensing in taste receptors, cerebrospinal fluid chemosensitivity, and calcium signaling. Its N-terminal domain plays a role in protein-protein interactions, channel assembly, and regulatory functions.
This antibody is commonly used in research to detect endogenous PKD2L1 expression via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). It is often raised in hosts such as rabbits or mice, with reactivity confirmed in human, mouse, or rat samples depending on the product. Validation typically includes knockout controls to ensure specificity.
Research applications focus on elucidating PKD2L1's role in sensory systems, ion homeostasis, and diseases like cancer or neurological disorders. By targeting the N-terminal region, the antibody helps distinguish full-length PKD2L1 from truncated isoforms, aiding studies on its structure-function relationships, cellular localization, and interaction partners. Proper validation remains critical due to potential cross-reactivity with homologous proteins in the TRPP family.
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