| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Coiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial, Protein N27C7-4, CHCHD10, C22orf16 |
| Entrez GeneID | 400916 |
| WB Predicted band size | 14.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CHCHD10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CHCHD10. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CHCHD10(N-term)抗体的3篇参考文献示例(注：文献为虚拟示例，实际引用需核实)：
1. **文献名称**：*"CHCHD10 N-terminal antibody reveals mitochondrial localization and disease-associated mutations"*
**作者**：A. Müller et al.
**摘要**：研究利用CHCHD10 N端特异性抗体，证实了该蛋白在线粒体内的定位，并发现ALS患者中特定突变导致其线粒体分布异常，影响嵴结构稳定性。
2. **文献名称**：*"Immunoblotting analysis of CHCHD10 in neurodegenerative disorders using a novel N-terminal antibody"*
**作者**：B. Chen et al.
**摘要**：开发了一种高特异性CHCHD10 N端抗体，通过免疫印迹和免疫组化验证其在额颞叶痴呆患者脑组织中的表达下降，提示蛋白功能缺失与疾病相关。
3. **文献名称**：*"CHCHD10 interacts with OPAL1 and regulates cristae morphology: Insights from antibody-based imaging"*
**作者**：K. Tanaka et al.
**摘要**：使用N端抗体进行共聚焦显微镜观察，发现CHCHD10与线粒体膜蛋白OPAL1互作，突变体导致线粒体嵴结构紊乱，加剧细胞凋亡。
(注：实际文献需通过PubMed或Google Scholar以关键词“CHCHD10 antibody N-term”检索。)
The CHCHD10 (N-term) antibody is a tool used to detect the N-terminal region of the CHCHD10 (coiled-coil-helix-coiled-coil-helix domain-containing protein 10), a mitochondrial protein implicated in cellular energy metabolism, oxidative stress response, and mitochondrial dynamics. CHCHD10 belongs to the CHCHD protein family, characterized by twin CHCH domains that facilitate protein-protein interactions and mitochondrial membrane localization. It plays a role in maintaining mitochondrial cristae structure, regulating apoptosis, and stabilizing respiratory chain complexes.
Mutations in the CHCHD10 gene are linked to neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), and mitochondrial myopathy. These mutations often disrupt mitochondrial function, leading to cellular stress and neuronal degeneration. The N-terminal region of CHCHD10 is critical for its stability, oligomerization, and interaction with partners like CHCHD2 and OPA1.
The CHCHD10 (N-term) antibody is widely used in research to study protein expression, localization, and pathological mechanisms in disease models. It is validated for techniques such as Western blotting, immunohistochemistry, and immunofluorescence, enabling the visualization of CHCHD10 in mitochondria-rich tissues like brain, muscle, and heart. Researchers also employ this antibody to explore how CHCHD10 dysfunction contributes to mitochondrial fragmentation, impaired oxidative phosphorylation, and neurodegeneration, offering insights into therapeutic targets for related diseases. Specificity is confirmed through knockout controls or siRNA-based validation to ensure minimal cross-reactivity.
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