| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Splicing factor, suppressor of white-apricot homolog, Splicing factor, arginine/serine-rich 8, Suppressor of white apricot protein homolog, SFSWAP, SFRS8, SWAP |
| Entrez GeneID | 6433 |
| WB Predicted band size | 104.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SFRS8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SFRS8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于SFRS8(N-term)抗体的3篇模拟参考文献示例，供参考：
---
1. **文献名称**：*SRSF8 regulates alternative splicing in human cell lines through N-terminal interactions*
**作者**：Johnson M, et al.
**摘要**：本研究利用针对SRSF8蛋白N端的特异性抗体(SFRS8 N-term抗体)，通过Western blot和免疫共沉淀技术，揭示了SRSF8在HeLa细胞中调控多种前体mRNA选择性剪接的分子机制。抗体特异性通过siRNA敲低实验验证。
2. **文献名称**：*Development and validation of a novel SFRS8 antibody for neurodegenerative disease studies*
**作者**：Chen L, et al.
**摘要**：报道了一种新型SFRS8(N-term)抗体的开发，通过免疫组化(IHC)和免疫荧光(IF)在小鼠脑组织中验证其特异性，并发现SRSF8在阿尔茨海默病模型中表达异常，提示其与tau蛋白异常剪接相关。
3. **文献名称**：*SFRS8 promotes tumor metastasis via EMT pathway activation: Evidence from antibody-based assays*
**作者**：Wang Y, et al.
**摘要**：使用SFRS8(N-term)抗体在乳腺癌细胞系(MCF-7、MDA-MB-231)中检测蛋白表达，结合CRISPR-Cas9基因编辑技术，证明SRSF8通过上调上皮-间质转化(EMT)相关基因促进肿瘤转移。
---
**备注**：以上文献为示例性内容，实际引用需根据具体研究检索真实文献(可通过PubMed或Google Scholar搜索关键词如“SRSF8 antibody”、“SFRS8 N-terminal”或结合疾病/功能关键词)。若需真实文献，建议补充具体研究背景或应用场景。
The SFRS8 (N-term) antibody targets the N-terminal region of Splicing Factor Arginine/Serine-Rich 8 (SFRS8), also known as SRSF8. a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors. SR proteins are critical in constitutive and alternative splicing, facilitating spliceosome assembly by binding exonic or intronic splicing enhancers. SFRS8 contains an N-terminal RNA recognition motif (RRM) for RNA binding and a C-terminal RS domain rich in arginine-serine dipeptides, which mediates protein-protein interactions and subcellular localization.
This antibody is commonly used to detect SFRS8 expression, localization, and function in studies of mRNA processing, particularly in investigating its role in splice site selection, mRNA export, and translation. Dysregulation of SFRS8 has been implicated in cancers and neurological disorders, making the antibody a tool for exploring disease mechanisms. Validated applications include Western blotting, immunofluorescence, and immunoprecipitation in human, mouse, or rat samples, depending on the antibody's host species (e.g., rabbit, mouse) and clonality.
Research using SFRS8 (N-term) antibodies has helped elucidate its interaction with other splicing regulators and its response to cellular stressors, such as DNA damage or metabolic changes, underscoring its importance in maintaining RNA homeostasis. Proper controls (e.g., knockout cells) are essential to confirm specificity due to potential cross-reactivity with homologous SR proteins.
×
