| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Rho GTPase-activating protein 36, ARHGAP36 |
| Entrez GeneID | 158763 |
| WB Predicted band size | 61.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RP13-102H20.1/ARHGAP36 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 373-402 amino acids from the C-terminal region of human RP13-102H20.1/ARHGAP36. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及ARHGAP36抗体的参考文献(均为虚构示例，实际文献需通过PubMed等数据库验证)：
1. **文献名称**：ARHGAP36 regulates Hedgehog signaling through primary cilia dynamics
**作者**：Smith J, et al.
**摘要**：研究发现ARHGAP36通过调控RhoA活性影响纤毛形成，利用特异性抗体证实其在小鼠胚胎神经管中的亚细胞定位，揭示其在Hedgehog信号传导中的新机制。
2. **文献名称**：ARHGAP45B/ARHGAP36 duplication promotes tumorigenesis in glioblastoma
**作者**：Chen L, et al.
**摘要**：通过Western blot和免疫荧光(使用ARHGAP36单克隆抗体)证明ARHGAP36在胶质母细胞瘤中高表达，并通过PI3K/AKT通路促进肿瘤侵袭，提示其作为潜在治疗靶点。
3. **文献名称**：Developmental expression profiling of ARHGAP36 in murine tissues
**作者**：Wang Y, et al.
**摘要**：利用兔多克隆ARHGAP36抗体进行组织染色，发现该蛋白在小鼠心脏、骨骼肌发育中动态表达，提示其参与肌细胞分化调控。
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提示：实际文献检索建议使用关键词"ARHGAP36 antibody"或"ARHGAP45B"(别名)，并筛选包含实验验证(如KO验证、定位分析)的研究。部分研究可能未直接命名"RP13-102H20.1"，需注意基因编号统一性。
The RP13-102H20.1/ARHGAP36 antibody targets the protein product of the ARHGAP36 gene, a member of the Rho GTPase-activating protein (RhoGAP) family. RhoGAPs regulate Rho GTPases, which are critical for cytoskeletal dynamics, cell motility, and signal transduction. ARHGAP36 is implicated in suppressing RhoA/ROCK signaling by enhancing GTP hydrolysis, thereby influencing cell shape, proliferation, and differentiation. Dysregulation of ARHGAP36 has been linked to developmental disorders and cancers, particularly due to its role in modulating Hedgehog (Hh) signaling pathways, where it may stabilize GLI transcription factors to drive oncogenesis.
The antibody is typically generated using immunogens such as recombinant protein fragments or synthetic peptides corresponding to specific epitopes within ARHGAP36. It is widely used in techniques like Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF) to study ARHGAP36 expression, localization, and function in cellular and tissue contexts. Validated applications often include detecting endogenous ARHGAP36 in human or mouse models, with specificity confirmed via knockout controls or siRNA-mediated silencing. Research utilizing this antibody has expanded insights into ARHGAP36's roles in neural development, tumor progression, and ciliopathies, highlighting its potential as a therapeutic target. Commercial suppliers provide detailed validation data, including host species (e.g., rabbit, mouse), clonality (polyclonal/monoclonal), and reactivity across species.
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