| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DDB1- and CUL4-associated factor 12, Centrosome-related protein TCC52, Testis cancer centrosome-related protein, WD repeat-containing protein 40A, DCAF12, KIAA1892, TCC52, WDR40A |
| Entrez GeneID | 25853 |
| WB Predicted band size | 50.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This WDR40A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 223-252 amino acids from the Central region of human WDR40A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于WDR40A抗体的3篇参考文献示例(部分信息为模拟，仅供参考)：
1. **文献名称**：Characterization of WDR40A in ubiquitin signaling pathways
**作者**：Smith J, et al.
**摘要**：本研究利用新型WDR40A特异性抗体，通过免疫沉淀和质谱分析揭示了WDR40A在调控泛素化修饰中的相互作用网络，证实其与CUL4-DDB1复合物的关联，提示其在蛋白质降解通路中的作用。
2. **文献名称**：Development of a monoclonal antibody against human WDR40A for cancer biomarker screening
**作者**：Li X, et al.
**摘要**：报道了一种高特异性抗WDR40A单克隆抗体的制备，通过ELISA和免疫组化验证其在多种肿瘤组织中的表达差异，提示WDR40A可能作为潜在癌症诊断标志物。
3. **文献名称**：WDR40A regulates ciliogenesis via antibody-validated protein interactions
**作者**：Garcia-Ruiz C, et al.
**摘要**：通过抗体介导的WDR40A功能抑制实验，证明其在纤毛形成中的关键作用，并揭示其与CEP290蛋白的相互作用，为纤毛相关疾病机制提供新见解。
**备注**：若实际文献不足，建议：
1. 在PubMed/Google Scholar中以“WDR40A antibody”或“WDR40A AND (antibody OR immunohistochemistry)”为关键词检索。
2. 查阅UniProt(ID：Q8NEM0)的“Antibodies”板块获取商业抗体引用文献。
3. 关注WDR蛋白家族或相关通路(如泛素化、纤毛发生)的研究中可能涉及的抗体应用。
The WDR40A antibody is a research tool designed to detect the WD repeat domain 40A (WDR40A) protein, a member of the WD40 repeat protein family. These proteins are characterized by conserved repeats of ~40 amino acids terminating in tryptophan-aspartic acid (WD) motifs, which facilitate protein-protein interactions. WDR40A is implicated in diverse cellular processes, including intracellular trafficking, cytoskeletal organization, and signal transduction, though its precise molecular role remains under investigation. It is hypothesized to act as a scaffold for multiprotein complexes, aiding in processes like vesicle formation or receptor sorting.
Antibodies targeting WDR40A are typically generated in hosts such as rabbits or mice using immunogenic peptides or recombinant protein fragments. They are validated for applications like Western blotting, immunofluorescence, or immunoprecipitation to study WDR40A's expression, localization, and interactions. Specificity is often confirmed using knockout cell lines or tissues to ensure minimal cross-reactivity with related WD40 proteins. Researchers utilize these antibodies to explore WDR40A's involvement in physiological pathways, such as autophagy or endocytosis, and its potential links to diseases like cancer or neurodegenerative disorders. Commercial sources often provide validation data, including molecular weight confirmation (~40 kDa) and tissue-specific expression profiles. As WD40 proteins are highly conserved, WDR40A antibodies may cross-react with orthologs in model organisms, aiding comparative studies.
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