| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA ligase 3, DNA ligase III, Polydeoxyribonucleotide synthase [ATP] 3, LIG3 |
| Entrez GeneID | 3980 |
| WB Predicted band size | 112.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This LIG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 793-822 amino acids from the C-terminal region of human LIG3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于LIG3抗体的3篇参考文献示例(文献信息为模拟虚构，仅供参考)：
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1. **文献名称**: *DNA Ligase III as a Key Component of Repair Pathways in Mammalian Cells*
**作者**: Smith A, et al.
**摘要**: 研究揭示了LIG3在哺乳动物细胞碱基切除修复(BER)中的核心作用，通过特异性抗体进行免疫沉淀和Western blot分析，证实LIG3与XRCC1蛋白的相互作用对维持基因组稳定性至关重要。
2. **文献名称**: *A Novel Monoclonal Antibody for Detecting LIG3 in Human Cancer Tissues*
**作者**: Chen L, et al.
**摘要**: 开发了一种高特异性抗人LIG3的单克隆抗体，验证其在免疫组织化学(IHC)中的应用，发现LIG3在多种癌症组织中表达上调，提示其可能作为肿瘤治疗的潜在靶点。
3. **文献名称**: *LIG3 Deficiency and Mitochondrial DNA Repair Defects*
**作者**: Johnson R, et al.
**摘要**: 利用LIG3敲除小鼠模型及特异性抗体检测，证明LIG3在线粒体DNA修复中的独特功能，其缺失导致线粒体功能障碍和细胞凋亡增加，为相关代谢疾病机制提供新见解。
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**注**：以上文献为示例，实际引用时请通过PubMed、Web of Science等平台检索真实论文。如需具体文献协助，可提供更详细的研究方向进一步筛选。
**Background of LIG3 Antibody**
The LIG3 antibody targets DNA ligase III (LIG3), a key enzyme encoded by the *LIG3* gene in humans. LIG3 plays a critical role in DNA repair mechanisms, particularly in the base excision repair (BER) pathway and single-strand break repair (SSBR), where it seals nicks in DNA through its catalytic ligase activity. Unlike other DNA ligases, LIG3 uniquely interacts with XRCC1 (X-ray repair cross-complementing protein 1), a scaffold protein essential for coordinating repair processes. This interaction stabilizes LIG3 and enhances its recruitment to DNA damage sites.
LIG3 exists in two isoforms: a nuclear form involved in DNA repair and a mitochondrial form crucial for maintaining mitochondrial DNA (mtDNA) integrity. Its mitochondrial role is vital for cellular energy production and apoptosis regulation. Dysregulation of LIG3 has been linked to genomic instability, cancer progression, and neurodegenerative disorders.
LIG3 antibodies are widely used in research to study LIG3 expression, subcellular localization, and function in DNA repair pathways. They are essential tools in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Specific antibodies can distinguish between nuclear and mitochondrial isoforms, aiding investigations into tissue-specific or stress-induced expression patterns. Additionally, LIG3 antibodies help explore therapeutic targets, such as chemoresistance in cancer, where LIG3 overexpression may impair treatment efficacy.
Overall, LIG3 antibodies are pivotal for understanding DNA repair mechanisms, mitochondrial biology, and their implications in disease pathogenesis.
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