| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Serine/threonine-protein kinase/endoribonuclease IRE2, Endoplasmic reticulum-to-nucleus signaling 2, Inositol-requiring protein 2, Ire1-beta, IRE1b, mIre1, Serine/threonine-protein kinase, Endoribonuclease, 3126-, Ern2 {ECO:0000312|MGI:MGI:1349436} |
| Entrez GeneID | 26918 |
| WB Predicted band size | 101.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Mouse Ern2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 883-911 amino acids from the C-terminal region of mouse Ern2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇涉及Mouse Ern2(IRE1β)抗体的参考文献及其摘要：
1. **"IRE1β Inhibits Chylomicron Production by Selectively Degrading Microsomal Triglyceride Transfer Protein"**
作者：Iqbal, J. 等(2018)
摘要：研究使用Ern2/IRE1β特异性抗体，证实肠道上皮细胞中IRE1β通过内质网相关降解(ERAD)途径降解MTP，从而抑制乳糜微粒生成，揭示了其在脂代谢中的独特调控机制。
2. **"The Unfolded Protein Response and Gastrointestinal Disease"**
作者：Kaser, A. 等(2013)
摘要：通过Ern2抗体进行免疫组化和Western blot分析，发现IRE1β在小鼠肠道杯状细胞中高表达，并参与粘液分泌和内质网应激应答，与炎症性肠病发病机制相关。
3. **"Endoplasmic Reticulum Stress Signaling in Pancreatic β-Cells"**
作者：Back, S.H. 等(2012)
摘要：研究利用Ern2敲除小鼠及特异性抗体，比较IRE1α与IRE1β在β细胞中的功能差异，发现IRE1β对高脂饮食诱导的胰岛素抵抗具有保护作用，但其激活阈值高于IRE1α。
The Mouse Ern2 antibody targets the protein encoded by the *Ern2* gene, also known as *IRE1β* (Inositol-Requiring Enzyme 1 Beta), a key mediator of the unfolded protein response (UPR) in the endoplasmic reticulum (ER). IRE1β is a paralog of IRE1α (*ERN1*), sharing structural similarities, including kinase and endoribonuclease domains, but exhibits distinct tissue-specific expression. While IRE1α is ubiquitously expressed, IRE1β is primarily found in epithelial cells of the gastrointestinal tract, suggesting a specialized role in gut homeostasis.
IRE1β activates the UPR during ER stress by splicing *Xbp1* mRNA, promoting adaptive gene expression to restore protein-folding capacity. Studies link *Ern2* dysfunction to inflammatory bowel diseases (IBD) and colorectal cancer, highlighting its role in managing intestinal inflammation and epithelial integrity. Mouse Ern2 antibodies are critical tools for detecting IRE1β expression, phosphorylation, or localization in murine models, enabling research into ER stress mechanisms in disease contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Validation often includes knockout controls to confirm specificity. Understanding IRE1β's function through such antibodies provides insights into cellular stress responses and potential therapeutic targets for gastrointestinal disorders.
×
