| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cyclin-dependent kinase-like 4, Cdkl4, Gm942 |
| Entrez GeneID | 381113 |
| WB Predicted band size | 39.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Mouse Cdkl4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272-299 amino acids from the C-terminal region of mouse Cdkl4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于小鼠CDKL4抗体的模拟参考文献示例(请注意这些为假设文献，建议通过学术数据库验证真实性和获取详细信息)：
1. **文献名称**: "Characterization of Cdkl4 Expression in Mouse Brain Using a Novel Polyclonal Antibody"
**作者**: Yamamoto A, et al.
**摘要**: 本研究开发了一种兔源多克隆抗体，用于检测小鼠CDKL4蛋白。通过Western blot在脑组织裂解液中验证了抗体的特异性，并利用免疫组化揭示了CDKL4在小鼠海马神经元中的高表达，提示其可能参与突触可塑性调控。
2. **文献名称**: "CDKL4 Kinase Activity in Cell Cycle Progression: Insights from Knockout Mouse Models"
**作者**: Chen L, et al.
**摘要**: 文章通过CRISPR构建Cdkl4基因敲除小鼠模型，采用定制抗体进行免疫沉淀和激酶活性分析，发现CDKL4通过磷酸化下游靶点调控G1/S期转换，抗体特异性经敲除组织Western blot确认。
3. **文献名称**: "Tissue-Specific Distribution of CDKL4 in Mouse Embryonic Development"
**作者**: Gupta S, et al.
**摘要**: 利用商业抗小鼠CDKL4抗体(货号AB123. XYZ公司)，通过免疫荧光和流式细胞术绘制了CDKL4在胚胎期小鼠心脏和神经嵴细胞的动态表达图谱，提示其在器官发育中的潜在作用。
4. **文献名称**: "Validation of Anti-CDKL4 Antibodies for Proteomic Studies in Murine T Cells"
**作者**: Müller R, et al.
**摘要**: 系统评估了三种市售CDKL4抗体的交叉反应性，发现仅克隆号4A7的小鼠单抗在流式细胞术中特异性识别活化T细胞的CDKL4.为免疫代谢研究提供可靠工具。
**建议**：实际研究中请通过PubMed、Web of Science等平台，以关键词“CDKL4 antibody mouse”检索最新文献，并优先选择经基因敲除验证或详细方法学描述的研究。
**Background of Mouse CDKL4 Antibody**
The mouse CDKL4 (Cyclin-dependent kinase-like 4) antibody is a tool designed to detect and study the CDKL4 protein, a member of the CDKL (CDK-like) kinase family. CDKL4 shares structural homology with cyclin-dependent kinases but exhibits distinct functional roles, primarily implicated in cellular signaling, transcriptional regulation, and cell cycle modulation. Though less characterized than its homolog CDKL5 (linked to neurodevelopmental disorders), CDKL4 is believed to interact with substrates involved in neuronal development and synaptic plasticity. Its expression has been observed in various tissues, with notable presence in the brain, suggesting potential roles in CNS function.
Mouse CDKL4 antibodies are typically developed using immunogens derived from specific epitopes of the murine protein, ensuring species-specific reactivity. These antibodies are validated for applications such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). Specificity is often confirmed using knockout controls or siRNA-mediated silencing.
Research utilizing CDKL4 antibodies aims to elucidate its involvement in developmental processes, disease mechanisms (e.g., neurological disorders or cancer), and signaling pathways. As CDKL4's precise biological functions remain under investigation, these antibodies serve as critical reagents for uncovering its molecular interactions and therapeutic potential.
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