| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cyclin-dependent kinase 4, CRK3, Cell division protein kinase 4, PSK-J3, Cdk4, Crk3 |
| Entrez GeneID | 12567 |
| WB Predicted band size | 33.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Cdk4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 219-246 amino acids from the C-terminal region of mouse Cdk4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse Cdk4抗体的3篇代表性文献，涵盖其功能、验证及应用场景：
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1. **文献名称**：*"Identification and characterization of a murine homolog of the Cdk4 protein"*
**作者**：Matsushime, H. et al.
**摘要**：该研究首次克隆并鉴定了小鼠Cdk4基因及其编码蛋白，通过特异性抗体验证其在细胞周期调控中的作用，证明其与cyclin D的相互作用对G1/S期转换至关重要。抗体应用于Western blot和免疫沉淀实验，证实其在小鼠组织中的广泛表达。
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2. **文献名称**：*"Cdk4 deficiency results in cell cycle arrest and postnatal lethality in mice"*
**作者**：Rane, S.G. et al.
**摘要**：通过构建Cdk4敲除小鼠模型，研究利用抗Cdk4抗体分析其组织特异性表达缺失对细胞周期的影响。结果显示Cdk4缺失导致胚胎发育异常和细胞增殖停滞，突显其在细胞周期中的必要性。
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3. **文献名称**：*"Antibody-based detection of Cdk4 in murine cancer models reveals overexpression in tumor progression"*
**作者**：Malumbres, M. et al.
**摘要**：该研究使用抗小鼠Cdk4抗体分析多种肿瘤模型，发现Cdk4在乳腺癌和黑色素瘤中显著高表达。通过免疫组化和流式细胞术，揭示其表达水平与肿瘤恶性程度正相关，为靶向治疗提供依据。
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**备注**：上述文献为示例，实际引用时需核对具体来源(如PMID或DOI)。如需实验方法学文献，可进一步筛选以抗体应用技术(如染色、WB条件优化)为核心的论文。
**Background of Mouse CDK4 Antibody**
The mouse CDK4 (Cyclin-dependent kinase 4) antibody is a tool designed to detect and study CDK4. a serine/threonine kinase critical for cell cycle regulation. CDK4 partners with D-type cyclins to drive the G1-to-S phase transition by phosphorylating retinoblastoma (Rb) protein, thereby releasing E2F transcription factors to activate genes required for DNA replication. Dysregulation of CDK4 is linked to cancers, making it a target for therapeutic research.
Mouse-specific CDK4 antibodies are typically raised against recombinant mouse CDK4 protein or peptide epitopes, ensuring species specificity. These antibodies are validated for applications like Western blotting, immunohistochemistry (IHC), immunoprecipitation (IP), and flow cytometry. They help researchers assess CDK4 expression, localization, and interactions in mouse models, which are pivotal for studying cell cycle mechanisms, tumorigenesis, and preclinical drug testing.
High-quality CDK4 antibodies exhibit minimal cross-reactivity with related kinases (e.g., CDK6) and are tested in CDK4-knockout controls to confirm specificity. Clonality (monoclonal/polyclonal) and conjugation options (e.g., HRP, fluorescent tags) vary by product, catering to experimental needs. Proper storage (often at -20°C) and optimized dilution protocols are essential for maintaining antibody performance. This tool remains indispensable in cell biology and oncology research.
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