| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transient receptor potential cation channel subfamily M member 4, hTRPM4, Calcium-activated non-selective cation channel 1, Long transient receptor potential channel 4, LTrpC-4, LTrpC4, Melastatin-4, TRPM4, LTRPC4 |
| Entrez GeneID | 54795 |
| WB Predicted band size | 134.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TRPM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1121-1149 amino acids from the C-terminal region of human TRPM4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TRPM4抗体的3篇参考文献示例(注：文献为虚构示例，仅用于格式参考)：
1. **文献名称**：*TRPM4 Channel Expression in Human Atrial Cardiomyocytes and Its Role in Arrhythmias*
**作者**：Zhang L, et al.
**摘要**：本研究通过免疫组化和Western blot技术，利用TRPM4特异性抗体证实其在人心房肌细胞中的高表达，并发现TRPM4活性异常与钙离子紊乱及房颤发生密切相关。
2. **文献名称**：*TRPM4 as a Potential Biomarker in Glioblastoma: Immunohistochemical Analysis*
**作者**：Smith J, et al.
**摘要**：通过TRPM4抗体对胶质母细胞瘤组织样本进行染色，发现TRPM4蛋白表达水平与肿瘤侵袭性呈正相关，提示其可能作为治疗靶点或预后指标。
3. **文献名称**：*Role of TRPM4 in T-Cell Activation and Autoimmune Response*
**作者**：Kim S, et al.
**摘要**：采用TRPM4抗体阻断实验结合流式细胞术，揭示TRPM4通过调控钙信号影响T细胞活化和自身免疫疾病进程，为免疫调节机制提供新见解。
(注：实际文献需通过PubMed、Google Scholar等平台以关键词“TRPM4 antibody”或“TRPM4 immunolocalization”检索获取。)
TRPM4 (Transient Receptor Potential Melastatin 4) is a calcium-activated non-selective cation channel that plays a critical role in regulating cellular electrical activity and calcium signaling. It primarily conducts monovalent cations like Na⁺ and K⁺ but is impermeable to divalent cations such as Ca²⁺. TRPM4 is involved in diverse physiological processes, including cardiac action potential shaping, immune cell activation, and neuronal signaling. Dysregulation of TRPM4 has been linked to cardiovascular diseases, immune disorders, and cancer, making it a target for therapeutic exploration.
TRPM4 antibodies are essential tools for studying the expression, localization, and function of the TRPM4 protein. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect TRPM4 in tissues or cultured cells. High-quality antibodies specifically recognize distinct epitopes on TRPM4. which consists of six transmembrane domains and cytoplasmic N- and C-termini. Validation of antibody specificity is crucial, as TRPM4 shares structural homology with other TRP channels, potentially leading to cross-reactivity. Knockout models or siRNA-mediated knockdown are often employed to confirm antibody reliability.
In research, TRPM4 antibodies have helped uncover its role in pathologies. For instance, upregulated TRPM4 in certain cancers correlates with metastasis, while mutations in TRPM4 are associated with cardiac conduction disorders. Despite their utility, challenges remain in developing antibodies that distinguish between splice variants or post-translationally modified forms. Ongoing efforts aim to refine these tools to advance mechanistic studies and therapeutic targeting of TRPM4-related diseases.
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