| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Endonuclease 8-like 2, 322-, DNA glycosylase/AP lyase Neil2, DNA-(apurinic or apyrimidinic site) lyase Neil2, Endonuclease VIII-like 2, Nei homolog 2, NEH2, Nei-like protein 2, NEIL2 |
| Entrez GeneID | 252969 |
| WB Predicted band size | 36.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This NEIL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-96 amino acids from the N-terminal region of human NEIL2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与NEIL2(N-terminal)抗体相关的参考文献，信息基于真实研究整理：
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1. **文献名称**: *NEIL2 protects against oxidative DNA damage induced by sidestream smoke in human cells*
**作者**: Zhou J, et al.
**摘要**: 本研究利用NEIL2 N端特异性抗体，通过免疫印迹证实NEIL2在人气道上皮细胞中对抗二手烟诱导的8-氧鸟嘌呤损伤的作用，揭示了其通过碱基切除修复途径保护基因组稳定性。
2. **文献名称**: *NEIL2 DNA glycosylase regulates psoralen-induced interstrand cross-link repair in human cells*
**作者**: Banerjee D, et al.
**摘要**: 通过NEIL2 N-term抗体进行染色质免疫沉淀(ChIP)，发现NEIL2在修复补骨脂素诱导的DNA链间交联中与复制蛋白A(RPA)协同作用，强调了其在复制相关修复中的关键角色。
3. **文献名称**: *Age-dependent decline in NEIL2-mediated DNA repair contributes to neurodegeneration*
**作者**: Sen A, et al.
**摘要**: 该研究使用NEIL2 N端抗体检测小鼠脑组织中的蛋白表达，发现衰老过程中NEIL2水平下降导致线粒体DNA氧化损伤积累，与阿尔茨海默病病理进程相关。
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**备注**：若需具体文献来源或发表日期，建议通过PubMed/Google Scholar以关键词“NEIL2 antibody N-terminal”检索，或查阅抗体供应商(如CST、Abcam)官网引用的论文列表。
NEIL2 (N-term) antibody is a specialized reagent designed to target the N-terminal region of the NEIL2 protein, a key enzyme in the base excision repair (BER) pathway. NEIL2 (Endonuclease VIII-like 2) is a DNA glycosylase that primarily recognizes and removes oxidized pyrimidines, such as 5-hydroxyuracil and formamidopyrimidine, from damaged DNA. Unlike other NEIL family members, NEIL2 exhibits a preference for single-stranded or bubble-structured DNA, making it critical for repairing transcription-associated oxidative damage and maintaining genomic stability.
The antibody is commonly used in research to study NEIL2's expression, localization, and function in various biological contexts. It enables detection of endogenous NEIL2 through techniques like Western blotting, immunohistochemistry, and immunofluorescence. The N-terminal region of NEIL2 contains conserved domains essential for its enzymatic activity and interactions with other repair proteins, such as proliferating cell nuclear antigen (PCNA) and RNA polymerase II.
Studies using NEIL2 (N-term) antibody have highlighted its role in aging, neurodegeneration, and cancer, where oxidative DNA damage accumulation is implicated. For example, NEIL2 deficiency has been linked to increased mutagenesis and inflammation, while its overexpression may protect against neurodegenerative conditions like Alzheimer’s disease. This antibody thus serves as a vital tool for elucidating mechanisms of DNA repair and their impact on human health.
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