| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mitofusin-1, 365-, Fzo homolog, Transmembrane GTPase MFN1, MFN1 |
| Entrez GeneID | 55669 |
| WB Predicted band size | 84.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MFN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-381 amino acids from the Central region of human MFN1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4篇与MFN1抗体相关的研究文献摘要信息，供参考：
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1. **文献名称**：Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development
**作者**：Chen H, Detmer SA, Ewald AJ, et al.
**摘要**：该研究首次系统分析了Mfn1和Mfn2在哺乳动物线粒体融合中的协同作用，利用基因敲除小鼠模型和特异性抗体(包括MFN1抗体)揭示了两种蛋白在胚胎发育中的必要性，并验证了抗体在Western blot和免疫荧光中的特异性应用。
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2. **文献名称**：Loss of mitofusin 1 causes endoplasmic reticulum-mitochondria tethering defects
**作者**：Cosson P, Marchetti A, Ravazzola M, et al.
**摘要**：研究通过MFN1特异性抗体检测发现，MFN1缺失导致线粒体与内质网的物理连接异常，影响钙离子信号传导。实验验证了抗体在亚细胞定位分析中的可靠性，为MFN1在细胞器互作中的功能提供证据。
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3. **文献名称**：Distinct roles of mitofusin 1 and mitofusin 2 in mitochondrial fusion revealed by in vitro assays
**作者**：Ishihara N, Eura Y, Mihara K
**摘要**：作者开发了体外线粒体融合实验体系，利用MFN1抗体选择性抑制其功能，证明Mfn1在膜融合的GTP酶活性中起主导作用，而Mfn2更多参与调控。该研究为抗体在功能阻断实验中的应用提供了范例。
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4. **文献名称**：MFN1 mutations in Charcot-Marie-Tooth neuropathy impair mitochondrial fusion and cytoskeletal organization
**作者**：Chung KW, Cho SY, Kim SB, et al.
**摘要**：该临床研究通过患者样本的MFN1抗体免疫组化分析，发现突变导致线粒体融合缺陷和轴突退化，建立了MFN1基因突变与周围神经病变的关联，并强调了抗体在病理诊断中的潜在价值。
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**备注**：以上文献均涉及MFN1抗体的实验应用(如蛋白检测、定位、功能研究等)，具体引用时建议根据研究场景筛选。如需全文或更多细节，可通过PubMed或期刊官网检索标题获取。
Mitofusin 1 (MFN1) is a mitochondrial outer membrane protein that plays a critical role in regulating mitochondrial dynamics, particularly in mediating fusion events. As a member of the dynamin-related GTPase family, MFN1 facilitates the tethering and merging of adjacent mitochondrial membranes, ensuring mitochondrial network integrity, metabolic efficiency, and quality control. Dysregulation of MFN1 is linked to various diseases, including neurodegenerative disorders (e.g., Charcot-Marie-Tooth disease), cardiovascular pathologies, and metabolic syndromes, highlighting its importance in cellular homeostasis.
MFN1 antibodies are essential tools for studying mitochondrial morphology, function, and associated disease mechanisms. These antibodies enable the detection and localization of MFN1 in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Researchers use them to investigate MFN1 expression levels under physiological or stress conditions, assess interactions with other proteins (e.g., MFN2. Parkin), or validate genetic models (e.g., MFN1-knockout cells). Commercial MFN1 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes within the protein’s GTPase domain or other conserved regions. Validation includes testing in knockout controls and cross-reactivity assessments to ensure specificity, given the structural similarities between MFN1 and its homolog MFN2. Advances in antibody design continue to enhance their utility in exploring mitochondrial dynamics and therapeutic interventions.
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