| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Arrestin domain-containing protein 1, ARRDC1 |
| Entrez GeneID | 92714 |
| WB Predicted band size | 46.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This ARRDC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-164 amino acids from the Central region of human ARRDC1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下为3-4条关于ARRDC1抗体的参考文献示例(虚构示例，供参考)：
1. **文献名称**: "ARRDC1 regulates tumor suppression via Notch1 ubiquitination"
**作者**: Smith J, et al.
**摘要**: 研究通过ARRDC1抗体进行Western blot和免疫组化，发现ARRDC1通过泛素化降解Notch1抑制肿瘤生长，抗体验证了其在多种癌细胞系中的表达缺失。
2. **文献名称**: "ARRDC1-mediated endocytosis of IGF-1R in metabolic regulation"
**作者**: Chen L, et al.
**摘要**: 利用ARRDC1抗体进行免疫共沉淀(Co-IP)和免疫荧光，证明ARRDC1通过调控胰岛素样生长因子受体(IGF-1R)内吞影响脂肪细胞分化和糖代谢。
3. **文献名称**: "Role of ARRDC1 in breast cancer cell migration"
**作者**: Gupta R, et al.
**摘要**: 研究使用ARRDC1抗体检测乳腺癌组织中蛋白表达水平，发现低表达ARRDC1与细胞迁移增强相关，机制涉及整合素β1信号通路抑制。
4. **文献名称**: "ARRDC1 interacts with viral proteins to modulate infection"
**作者**: Wang Y, et al.
**摘要**: 通过ARRDC1抗体进行免疫印迹和免疫沉淀，揭示ARRDC1与HIV-1病毒蛋白Nef相互作用，调控病毒出芽过程。
(注：以上文献为模拟内容，实际文献需通过PubMed/Google Scholar等平台检索。)
ARRDC1 (Arrestin domain-containing protein 1) is a member of the α-arrestin family, which plays a role in regulating membrane protein trafficking, ubiquitination, and lysosomal degradation. It contains a conserved arrestin fold and PPxY motifs that mediate interactions with E3 ubiquitin ligases, such as NEDD4. facilitating substrate ubiquitination. ARRDC1 is involved in diverse cellular processes, including G protein-coupled receptor (GPCR) internalization, extracellular vesicle formation, and metabolic regulation. Studies link ARRDC1 to cancer progression, obesity, and immune responses, highlighting its dual roles as a tumor suppressor or promoter depending on context.
Antibodies targeting ARRDC1 are essential tools for investigating its expression, localization, and function. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study ARRDC1's tissue distribution (e.g., enriched in heart, liver, and skeletal muscle) and dynamic regulation under stress or disease conditions. Commercial ARRDC1 antibodies are typically raised against specific epitopes, such as human N-terminal or C-terminal regions, and validated using knockout controls or recombinant proteins. Reliable antibodies aid in exploring ARRDC1's interplay with signaling pathways (e.g., Hippo, Notch) and its potential as a therapeutic target in metabolic disorders or malignancies.
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