| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Nuclear receptor subfamily 1 group D member 2, Orphan nuclear hormone receptor BD73, Rev-erb alpha-related receptor, RVR, Rev-erb-beta, V-erbA-related protein 1-related, EAR-1R, NR1D2 |
| Entrez GeneID | 9975 |
| WB Predicted band size | 64.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This NR1D2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-294 amino acids from the Central region of human NR1D2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NR1D2抗体的参考文献示例(内容为虚构，仅作格式参考)：
1. **"NR1D2 regulates hepatic lipid metabolism through circadian transcription control"**
*作者：Zhang L, et al.*
摘要：本研究利用NR1D2特异性抗体，通过ChIP-seq和Western blot技术，揭示了NR1D2在肝脏脂代谢中的核心调控作用，并证实其与昼夜节律基因的相互作用。
2. **"Rev-Erbβ (NR1D2) antibody validation in human colorectal cancer tissues"**
*作者：Martinez R, et al.*
摘要：通过免疫组化及免疫荧光实验，验证了NR1D2抗体在结直肠癌组织中的特异性表达，发现NR1D2低表达与患者预后不良相关。
3. **"Development of a high-affinity monoclonal antibody for NR1D2 functional studies"**
*作者：Kim S, et al.*
摘要：报道了一种新型NR1D2单克隆抗体的开发，经流式细胞术和共聚焦显微镜验证，该抗体可特异性识别内源性NR1D2蛋白，适用于亚细胞定位研究。
4. **"NR1D2 modulates macrophage polarization via antibody-based pathway analysis"**
*作者：Chen X, et al.*
摘要：结合NR1D2抗体进行蛋白质相互作用筛选，发现其通过调控NF-κB通路影响巨噬细胞极化，为炎症性疾病机制提供新见解。
The NR1D2 antibody targets the nuclear receptor subfamily 1. group D, member 2 (NR1D2), also known as REV-ERBβ, a ligand-regulated transcription factor belonging to the nuclear receptor superfamily. NR1D2 is structurally characterized by a DNA-binding domain (DBD) and a ligand-binding domain (LBD), though it lacks a classical activation domain, functioning primarily as a transcriptional repressor. It binds to retinoic acid-related orphan receptor response elements (ROREs) in gene promoters, recruiting corepressors like HDAC3 to suppress target genes.
NR1D2 plays a critical role in regulating circadian rhythm by interacting with core clock components such as BMAL1 and CLOCK, forming a feedback loop essential for maintaining daily physiological cycles. Beyond circadian regulation, it influences metabolic pathways, including lipid metabolism, glucose homeostasis, and inflammation. Dysregulation of NR1D2 has been implicated in metabolic disorders, cancer, and neurodegenerative diseases, making it a potential therapeutic target.
NR1D2 antibodies are widely used in research to detect protein expression and localization via techniques like Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF). These tools help elucidate its tissue-specific roles, temporal expression patterns, and interactions with signaling pathways. Studies using NR1D2 antibodies have highlighted its differential expression in metabolic tissues (e.g., liver, adipose) and its crosstalk with other nuclear receptors (e.g., PPARs), advancing understanding of its physiological and pathological functions.
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