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Rabbit Polyclonal TCF12(N-term) Antibody

  • 中文名： TCF12 (N-term)抗体
  • 别    名： Transcription factor 12, TCF-12, Class B basic helix-loop-helix protein 20, bHLHb20, DNA-binding protein HTF4, E-box-binding protein, Transcription factor HTF-4, TCF12, BHLHB20, HEB, HTF4
货号: IPDX31540
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesTranscription factor 12, TCF-12, Class B basic helix-loop-helix protein 20, bHLHb20, DNA-binding protein HTF4, E-box-binding protein, Transcription factor HTF-4, TCF12, BHLHB20, HEB, HTF4
Entrez GeneID6938
WB Predicted band size73.0kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenThis TCF12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-84 amino acids from the N-terminal region of human TCF12.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于TCF12(N-term)抗体的3篇参考文献示例(注：文献信息为模拟示例，实际引用需核实)：

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1. **文献名称**: *TCF12 regulates cranial suture development through β-catenin interaction*

**作者**: Smith A, et al.

**摘要**: 本研究利用TCF12(N-term)抗体进行免疫沉淀和染色质分析，发现TCF12通过结合E-box序列调控颅骨缝发育，并证实其与β-catenin信号通路的相互作用。抗体特异性通过TCF12敲除细胞系的Western blot验证。

2. **文献名称**: *Aberrant TCF12 expression in glioblastoma correlates with patient prognosis*

**作者**: Chen L, et al.

**摘要**: 通过TCF12(N-term)抗体的免疫组化分析，发现胶质母细胞瘤中TCF12蛋白的异常核表达与患者生存率显著相关，提示其作为预后标志物的潜力。抗体特异性通过siRNA敲低实验验证。

3. **文献名称**: *Functional characterization of TCF12 mutations in syndromic craniosynostosis*

**作者**: Johnson R, et al.

**摘要**: 研究利用TCF12(N-term)抗体进行免疫荧光染色，揭示TCF12基因突变导致蛋白亚细胞定位异常，破坏成骨细胞分化，进而引发颅缝早闭综合征。抗体在患者组织样本中显示出高特异性。

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如需实际文献，建议通过PubMed或抗体供应商(如CST、Abcam)的产品引用列表查找具体研究。

背景信息

The TCF12 (N-term) antibody is designed to detect the N-terminal region of the Transcription Factor 12 (TCF12) protein, a member of the basic helix-loop-helix (bHLH) E-protein family. TCF12. also known as HEB, plays critical roles in regulating gene expression by forming homodimers or heterodimers with other bHLH proteins, such as TWIST1. to control cellular processes including differentiation, proliferation, and development. It is particularly important in neural crest cell migration, craniofacial development, and T-cell maturation. Dysregulation of TCF12 has been linked to congenital disorders like Saethre-Chotzen syndrome (craniosynostosis) and malignancies such as T-cell acute lymphoblastic leukemia (T-ALL). The antibody targets epitopes near the N-terminus, which may overlap with functional domains involved in protein dimerization or DNA binding. Researchers commonly use this antibody in techniques like Western blotting, immunoprecipitation, and immunohistochemistry to study TCF12 expression, localization, and interactions in both normal and pathological contexts. Validation typically includes knockout controls to confirm specificity. Its application aids in exploring TCF12's role in developmental biology, cancer mechanisms, and potential therapeutic targeting.

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