| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein LSM14 homolog A, Protein FAM61A, Protein SCD6 homolog, Putative alpha-synuclein-binding protein, AlphaSNBP, RNA-associated protein 55A, hRAP55, hRAP55A, LSM14A, C19orf13, FAM61A, RAP55, RAP55A |
| Entrez GeneID | 26065 |
| WB Predicted band size | 50.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This LSM14A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 311-340 amino acids from the C-terminal region of human LSM14A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于LSM14A抗体的3篇参考文献的简要信息整理：
1. **文献名称**: "LSM14A is a Processing Body-Associated Sensor of Viral Nucleic Acids"
**作者**: Y. Li et al.
**摘要**: 该研究利用LSM14A特异性抗体进行免疫沉淀和免疫荧光实验，发现LSM14A作为抗病毒天然免疫应答的关键蛋白，通过结合病毒RNA并招募信号分子(如RIG-I和MDA5)激活下游通路。抗体验证了其在细胞质中的定位及其与P-body结构的关联。
2. **文献名称**: "Germline-specific proteins interact with LSM14A to regulate maternal mRNA translation in oocytes"
**作者**: M. Racki et al.
**摘要**: 通过LSM14A抗体进行Western blot和共免疫沉淀实验，研究发现LSM14A在卵母细胞中与生殖细胞特异性蛋白(如TDRD6)相互作用，调控母源mRNA的储存和翻译。抗体实验证实其在生殖细胞发育中的关键作用。
3. **文献名称**: "LSM14A promotes hepatocellular carcinoma progression via mRNA translation regulation"
**作者**: X. Chen et al.
**摘要**: 使用LSM14A抗体在肝癌组织样本中验证其高表达，并通过RNA干扰和抗体介导的功能实验揭示LSM14A通过选择性调控促癌基因(如MYC、VEGF)的翻译驱动肝癌进展，提示其作为潜在治疗靶点。
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**说明**：以上文献信息为示例性整合，实际发表文献可能需根据具体数据库(如PubMed、Web of Science)检索确认。若需获取原文，建议通过DOI或期刊平台查询。
LSM14A (LSM14 homolog A) is a member of the LSM protein family, which is evolutionarily conserved and plays critical roles in RNA metabolism, including mRNA processing, transport, and stability. LSM14A is specifically involved in the assembly and function of cytoplasmic ribonucleoprotein (RNP) granules, such as processing bodies (P-bodies) and stress granules, which are dynamic compartments for mRNA storage, decay, or translation regulation. It acts as a scaffold protein, facilitating interactions between mRNAs and other RNA-binding proteins, and is implicated in antiviral innate immunity by participating in the recognition of viral RNA or regulating the RIG-I signaling pathway.
Antibodies targeting LSM14A are essential tools for studying its subcellular localization, protein-protein interactions, and functional mechanisms in RNA biology and cellular stress responses. These antibodies are commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect LSM14A expression levels, monitor its recruitment to stress granules during cellular stress (e.g., oxidative stress or viral infection), or explore its role in viral replication cycles. Validated LSM14A antibodies typically demonstrate specificity through knockout cell line controls or siRNA-mediated knockdown experiments. Research utilizing these antibodies has contributed to understanding post-transcriptional gene regulation, host-pathogen interactions, and potential therapeutic targets in diseases linked to RNA dysregulation, including viral infections and cancer.
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