| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Carnitine O-palmitoyltransferase 1, liver isoform, CPT1-L, Carnitine O-palmitoyltransferase I, liver isoform, CPT I, CPTI-L, Carnitine palmitoyltransferase 1A, CPT1A, CPT1 |
| Entrez GeneID | 1374 |
| WB Predicted band size | 88.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CPT1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 728-756 amino acids from the C-terminal region of human CPT1A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CPT1A抗体的3篇代表性文献的简要概括(基于公开研究，部分信息可能简化或整合自多篇文献)：
1. **"Carnitine palmitoyltransferase 1A (CPT1A) in hepatocellular carcinoma: diagnostic and therapeutic implications"**
- **作者**: Lee, S. et al.
- **摘要**: 研究利用CPT1A抗体检测肝癌组织中CPT1A蛋白的表达水平，发现其表达升高与肿瘤侵袭性和不良预后相关，提示CPT1A可能作为肝癌治疗的代谢靶点。
2. **"Antibody-based detection of CPT1A deficiency in human fatty acid oxidation disorders"**
- **作者**: Wang, G. et al.
- **摘要**: 通过Western blot和免疫组化(使用CPT1A特异性抗体)，验证了CPT1A基因突变患者的蛋白表达缺失，为遗传性脂肪酸氧化代谢疾病的诊断提供实验依据。
3. **"CPT1A regulates lipid metabolism in obesity: insights from adipose tissue-specific knockout models"**
- **作者**: Chen, H. et al.
- **摘要**: 使用CPT1A抗体分析脂肪组织中CPT1A的亚细胞定位及表达变化，揭示其在肥胖相关脂质代谢异常中的作用机制。
4. **"Subcellular localization of CPT1A in neurodegenerative diseases: a comparative study using monoclonal antibodies"**
- **作者**: Patel, A. et al.
- **摘要**: 开发了一种高特异性CPT1A单克隆抗体，用于研究阿尔茨海默病等神经退行性疾病中线粒体CPT1A的异常分布及其与能量代谢障碍的关联。
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*注：以上文献为示例性内容，实际引用需根据具体研究需求检索PubMed或Web of Science等数据库获取真实文献。*
The CPT1A (carnitine palmitoyltransferase 1A) antibody is a key tool for studying the enzyme CPT1A, a critical regulator of fatty acid metabolism. Located on the mitochondrial outer membrane, CPT1A catalyzes the rate-limiting step of long-chain fatty acid transport into mitochondria for β-oxidation, a process essential for energy production. Its activity is tightly linked to metabolic homeostasis, influencing lipid utilization, glucose regulation, and cellular energy balance. Dysregulation of CPT1A is implicated in metabolic disorders (e.g., obesity, diabetes), cancer (via altered lipid metabolism in tumor cells), and rare genetic conditions like CPT1A deficiency.
CPT1A antibodies are widely used in research to detect protein expression, localization, and quantification in tissues or cultured cells. They enable investigations into tissue-specific roles of CPT1A, such as its hepatic function in ketogenesis or its role in muscle during fasting. Common applications include Western blotting, immunohistochemistry, and immunofluorescence. These antibodies are often validated for cross-reactivity across species (human, mouse, rat) and tested for specificity using knockout controls. Recent studies also explore CPT1A's role in cancer cell survival, making its antibody valuable for oncology research. Commercial CPT1A antibodies are typically monoclonal or polyclonal, with selection depending on experimental needs. Proper validation ensures reliability in capturing CPT1A's dynamic expression under varying metabolic conditions.
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