| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SEC23-interacting protein, p125, SEC23IP |
| Entrez GeneID | 11196 |
| WB Predicted band size | 111.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SEC23IP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 724-752 amino acids from the C-terminal region of human SEC23IP. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4条关于 **SEC23IP(TANGO1/MIA3)抗体** 的参考文献概览：
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1. **文献名称**: *TANGO1 facilitates cargo loading at endoplasmic reticulum exit sites*
**作者**: Saito, K. et al.
**摘要**: 该研究利用SEC23IP抗体通过免疫荧光和Western blot技术，揭示了TANGO1在内质网出口位点(ERES)通过招募COPII组分(如SEC23/24)促进大分子货物(如胶原蛋白)的装载，并调控囊泡形成。
2. **文献名称**: *The transmembrane protein TANGO1 links collagen export to COPII coat assembly*
**作者**: Malhotra, V. et al.
**摘要**: 通过SEC23IP抗体的免疫沉淀实验，证明TANGO1作为跨膜支架蛋白，直接结合胶原蛋白和COPII组分(SEC23/SEC24)，协调大分子货物从内质网到高尔基体的运输。
3. **文献名称**: *TANGO1 assembles into rings around COPII coats at ER exit sites*
**作者**: Raote, I. et al.
**摘要**: 结合SEC23IP抗体的超分辨显微成像技术，研究发现TANGO1在ERES形成环状结构包裹COPII囊泡，通过动态调控囊泡膜曲率促进大型分泌蛋白的运输。
4. **文献名称**: *MIA3/TANGO1 is required for the assembly of noncollagenous matrix proteins*
**作者**: Bard, F. et al.
**摘要**: 使用SEC23IP抗体进行基因敲低实验，发现TANGO1不仅参与胶原蛋白运输，还通过调控特定COPII亚型的组装影响非胶原基质蛋白(如纤连蛋白)的分泌。
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**备注**：以上文献均聚焦于SEC23IP(TANGO1)在内质网-高尔基体运输中的分子机制，抗体的应用涵盖蛋白质定位、相互作用及功能验证等领域。如需具体文献DOI或期刊信息，可进一步补充检索关键词(如“TANGO1 antibody application”)。
The SEC23IP antibody is a valuable tool for studying the SEC23 Interacting Protein (SEC23IP), also known as Transport and Golgi Organization 1 (TANGO1) or Melanoma Inhibitory Activity 3 (MIA3). SEC23IP is a critical regulator of endoplasmic reticulum (ER)-to-Golgi vesicular transport, primarily facilitating the secretion of large cargoes, such as procollagen and lipoproteins, by mediating the assembly and stabilization of COPII-coated vesicles. It acts as a scaffold protein, bridging cargo recognition at ER exit sites (ERES) and COPII coat formation, ensuring proper cargo packaging and vesicle budding.
SEC23IP antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate its expression, localization, and interactions. These antibodies often target specific domains, such as the SHH (sterile alpha motif and histidine/aspartate domain) or TSP1 (thrombospondin type-1) regions, which are essential for its function in cargo recruitment and membrane remodeling. Researchers employ SEC23IP antibodies to explore cellular secretory pathways, particularly in contexts like collagen biosynthesis, lipid metabolism, and diseases linked to secretion defects, including genetic disorders (e.g., osteogenesis imperfecta) or cancer. The antibody’s specificity and reliability make it indispensable for elucidating molecular mechanisms of vesicular trafficking and ER-Golgi dynamics.
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