| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Hamartin, Tuberous sclerosis 1 protein, TSC1, KIAA0243, TSC |
| Entrez GeneID | 7248 |
| WB Predicted band size | 129.8kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | Purified His-tagged TSC1 protein(Fragment) was used to produced this monoclonal antibody. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TSC1抗体的3篇代表性文献，涵盖其在不同研究中的应用和机制分析：
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1. **文献名称**：*"TSC1 and TSC2: Critical elements of a phosphatidylinositol-3-kinase pathway that regulates cell size"*
**作者**：Potter CJ, Pedraza LG, Xu T
**摘要**：该研究揭示了TSC1-TSC2复合物通过抑制mTORC1信号通路调控细胞生长的机制。文中使用TSC1抗体进行免疫共沉淀实验，证实TSC1与TSC2的相互作用，并证明其在PI3K/Akt通路中的负调控作用。
2. **文献名称**：*"Tuberous sclerosis complex gene products regulate autophagy via mTOR-dependent and independent pathways"*
**作者**：Zhou X, Ikenoue T, Chen J
**摘要**：研究通过Western blot和免疫荧光技术(使用TSC1抗体)发现，TSC1/2缺失导致mTOR过度激活，同时影响自噬相关蛋白LC3的加工，表明TSC复合物在自噬调控中的双重作用。
3. **文献名称**：*"Antibody-based profiling of TSC1 expression in human tumors: Implications for mTOR-targeted therapies"*
**作者**：Guo Y, Chekaluk Y, Wu J
**摘要**：该研究开发了一种高特异性TSC1抗体，用于免疫组化分析多种肿瘤组织。结果显示TSC1表达缺失与mTOR抑制剂治疗敏感性相关，为临床生物标志物研究提供了工具支持。
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**说明**：以上文献示例聚焦于TSC1抗体的实验应用(如蛋白质互作验证、信号通路分析、临床检测)，可根据实际研究方向调整参考文献的侧重点。建议通过PubMed或Google Scholar以“TSC1 antibody”、“TSC1 immunohistochemistry”等关键词进一步筛选最新研究。
The TSC1 antibody is a crucial tool in studying the tuberous sclerosis complex (TSC) pathway, which regulates cell growth, proliferation, and metabolism. TSC1 (tuberous sclerosis complex 1), also known as hamartin, forms a heterodimeric complex with TSC2 (tuberin) to inhibit the mTOR (mechanistic target of rapamycin) signaling pathway. This complex acts as a tumor suppressor by integrating cellular signals, such as energy status, growth factors, and stress, to modulate mTORC1 activity. Dysregulation of TSC1/2 leads to mTOR hyperactivation, linked to tuberous sclerosis—a genetic disorder characterized by benign tumors in multiple organs—and cancers.
The TSC1 antibody specifically detects the TSC1 protein in various experimental applications, including Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). It aids in assessing TSC1 expression levels, subcellular localization, and post-translational modifications (e.g., phosphorylation), which influence its stability and function. Researchers use this antibody to explore TSC1's role in diseases like cancer, neurological disorders, and metabolic syndromes, as well as in evaluating therapeutic responses to mTOR inhibitors.
Available as monoclonal or polyclonal variants, TSC1 antibodies are validated for species reactivity (e.g., human, mouse, rat) and specificity. Proper controls, such as knockout cell lines, are essential to confirm antibody reliability. Their utility extends to both basic research and clinical diagnostics, advancing understanding of mTOR-related pathologies and targeted treatment strategies.
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