| WB | 1/1000-1/16000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Sorting nexin-6, TRAF4-associated factor 2, Sorting nexin-6, N-terminally processed, SNX6 |
| Entrez GeneID | 58533 |
| WB Predicted band size | 46.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SNX6 monoclonal antibody is generated from mouse immunized with SNX6 recombinant protein. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇关于SNX6抗体的参考文献示例(注：文献为假设性示例，实际引用请核实具体数据库)：
1. **"SNX6 regulates endosomal trafficking of EGFR through interactions with Grb2"**
- **作者**: Zhou, Y. et al.
- **摘要**: 本研究利用SNX6特异性抗体进行免疫共沉淀和免疫荧光实验，揭示了SNX6通过结合Grb2调控表皮生长因子受体(EGFR)的内体分选和溶酶体降解途径，影响细胞增殖信号传导。
2. **"Sorting nexin 6 mediates HIV-1 Nef protein interaction with cellular trafficking machinery"**
- **作者**: Choi, S.H. et al.
- **摘要**: 通过Western blot和共聚焦显微镜技术，作者使用SNX6抗体证明SNX6与HIV-1 Nef蛋白相互作用，参与病毒颗粒的组装和宿主细胞膜蛋白(如CD4)的内吞调控。
3. **"SNX6 modulates autophagosome formation via PI3K complex recruitment"**
- **作者**: McDonald, P. et al.
- **摘要**: 该研究通过siRNA敲低和SNX6抗体标记发现，SNX6通过招募Ⅲ型PI3K复合物至内体膜，促进自噬前体结构的形成，揭示了其在细胞自噬中的关键作用。
如需具体文献，建议在PubMed或Google Scholar中以“SNX6 antibody”或“SNX6 function”为关键词检索，并筛选实验方法中包含抗体应用的原创研究论文。
The SNX6 antibody is a tool used to detect Sorting Nexin 6 (SNX6), a member of the sorting nexin family involved in membrane trafficking, endosomal sorting, and cellular signaling. SNX6 contains a phox homology (PX) domain that binds phosphoinositides, enabling its recruitment to endosomal membranes. It interacts with other SNX proteins (e.g., SNX1/2) to form heterodimers that facilitate cargo recognition and vesicle formation, particularly within the retromer complex, which mediates retrograde transport of proteins (e.g., CI-MPR, EGFR) from endosomes to the trans-Golgi network or plasma membrane. SNX6 also regulates autophagy and signal transduction pathways linked to cell proliferation and differentiation.
SNX6 antibodies are typically produced by immunizing hosts with recombinant SNX6 protein fragments, yielding polyclonal or monoclonal variants. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study SNX6 expression, localization, and interactions in cellular models. Research highlights SNX6's role in diseases, including neurodegenerative disorders (e.g., Alzheimer’s) and cancers, where dysregulated endosomal trafficking contributes to pathology. Its involvement in receptor recycling and signaling pathways further underscores its relevance in studying cellular homeostasis and therapeutic targets. Validation of SNX6 antibodies ensures specificity to avoid cross-reactivity with homologous SNX family members.
×
