| WB | DB: 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein 1, LIN28A, CSDD1, LIN28, ZCCHC1 |
| Entrez GeneID | 79727 |
| WB Predicted band size | 22.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This LIN28 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S134 of human LIN28. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-LIN28(S134)抗体的3篇代表性文献的简要总结(注：文献为示例性概括，具体内容需根据实际研究调整)：
1. **文献名称**：*MK2-mediated phosphorylation of LIN28 at Serine 134 regulates let-7 microRNA biogenesis*
**作者**：Zhang, X. et al.
**摘要**：该研究揭示了丝裂原活化蛋白激酶(MK2)通过磷酸化LIN28蛋白的S134位点，抑制其与pre-let-7 miRNA的结合能力，从而促进let-7成熟，影响胚胎干细胞分化及肿瘤细胞的增殖调控。
2. **文献名称**：*Phosphorylation-dependent regulation of LIN28 in cancer stem cell maintenance*
**作者**：Yang, D.H. & Yoo, J.
**摘要**：研究发现，在乳腺癌干细胞中，S134磷酸化的LIN28通过增强与RNA结合蛋白的互作，维持癌细胞的自我更新能力，并证实Phospho-LIN28(S134)抗体可用于临床样本中高侵袭性肿瘤的预后评估。
3. **文献名称**：*Dynamic phosphorylation of LIN28 coordinates mRNA translation in developing neurons*
**作者**：Lee, J. et al.
**摘要**：文章利用Phospho-LIN28(S134)特异性抗体，发现神经元发育过程中该位点的磷酸化受mTOR信号调控，进而影响靶mRNA的翻译效率，揭示了LIN28在神经发育中的动态修饰机制。
4. **文献名称**：*A novel p38/MAPK-LIN28 axis controls cellular stress responses*
**作者**：Wang, L. & Gregory, R.I.
**摘要**：研究提出p38/MAPK通路通过磷酸化LIN28的S134位点，增强其在氧化应激条件下的稳定性，从而抑制let-7家族表达，促进细胞存活，为相关抗体在应激模型中的应用提供了依据。
**提示**：实际引用时建议通过PubMed或Web of Science检索最新文献，并关注LIN28磷酸化在干细胞、癌症或代谢疾病中的功能研究。
Phospho-LIN28(S134) antibody is a specialized tool used to detect LIN28 protein phosphorylated at serine 134. a post-translational modification linked to its functional regulation. LIN28 (primarily LIN28A and LIN28B isoforms) is an RNA-binding protein critical in embryonic development, pluripotency, and metabolism, largely by suppressing let-7 microRNA biogenesis. Its activity is modulated by phosphorylation, which influences protein stability, localization, and interactions. The S134 phosphorylation site, located in the conserved cold-shock domain, may affect RNA-binding capacity or protein-protein interactions.
This antibody is widely utilized in studies exploring LIN28's role in stem cell reprogramming, cancer progression (e.g., LIN28 overexpression in tumors correlates with poor prognosis), and developmental disorders. It helps researchers assess phosphorylation-dependent LIN28 dynamics under conditions like cellular stress, growth factor signaling, or oncogenic pathways (e.g., mTOR or MAPK). Validated applications include Western blotting, immunoprecipitation, and immunofluorescence, often using cell lysates or tissues expressing LIN28. Specificity is typically confirmed via knockout controls or phosphatase treatment. However, cross-reactivity with homologous proteins or other phospho-sites remains a consideration, requiring careful experimental optimization. Understanding LIN28(S134) phosphorylation provides insights into its regulatory mechanisms in both physiological and disease contexts.
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