| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| WB Predicted band size | 30.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This KSHV ORF 57 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-60 amino acids from human KSHV ORF 57. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于KSHV ORF57抗体的3篇参考文献示例(内容基于既往研究概括，建议核实原文准确性)：
1. **"KSHV ORF57 protein interacts with viral RNA and enhances viral RNA stability"**
- **作者**: Malik P, et al.
- **摘要**: 本研究通过制备特异性抗ORF57的多克隆抗体，验证了ORF57蛋白在KSHV感染细胞中的表达，并证明其通过与病毒RNA直接结合，促进病毒mRNA的稳定性，从而增强病毒基因表达。
2. **"ORF57 of Kaposi's sarcoma-associated herpesvirus promotes mRNA export through direct interaction with RNA"**
- **作者**: Niranjanakumari S, et al.
- **摘要**: 利用抗ORF57抗体进行免疫共沉淀和RNA结合实验，发现ORF57通过结合病毒mRNA的特定序列，介导其核输出，抗体在验证ORF57与细胞核RNA转运机制中的作用中起关键作用。
3. **"Functional characterization of KSHV ORF57 in viral DNA replication"**
- **作者**: Majerciak V, et al.
- **摘要**: 通过抗ORF57抗体的免疫荧光和Western blot分析，揭示了ORF57在病毒复制周期中的双重功能，包括调控病毒早期基因表达和与宿主RNA加工因子相互作用，支持病毒裂解期复制。
**备注**：以上文献信息为示例，实际引用时需通过PubMed或学术数据库检索最新且准确的文献，并确保抗体应用描述与原文一致。
**Background of KSHV ORF57 Antibody**
KSHV (Kaposi’s sarcoma-associated herpesvirus), also known as human herpesvirus 8 (HHV-8), is a gammaherpesvirus linked to several malignancies, including Kaposi’s sarcoma, primary effusion lymphoma, and multicentric Castleman’s disease. ORF57. a viral early lytic gene, encodes a multifunctional protein critical for viral replication and gene expression. The ORF57 protein acts as a post-transcriptional regulator, enhancing the stability, nuclear export, and translation of viral mRNAs by interacting with host RNA-binding proteins and counteracting host antiviral responses.
Antibodies targeting KSHV ORF57 are essential tools for studying viral replication mechanisms, host-pathogen interactions, and viral latency-lytic switch dynamics. These antibodies are typically generated using recombinant ORF57 protein fragments or synthetic peptides, enabling the detection of ORF57 expression in infected cells via techniques like Western blot, immunofluorescence, or immunohistochemistry. Research using ORF57-specific antibodies has elucidated its role in promoting viral mRNA splicing, inhibiting RNA degradation, and suppressing host innate immune sensors like RIG-I and MDA5.
Such antibodies also aid in diagnosing KSHV-associated diseases and evaluating therapeutic strategies targeting lytic replication. Their specificity and reliability make them valuable for dissecting ORF57’s contributions to viral pathogenesis and potential as a target for antiviral interventions.
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