| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Homeobox protein Hox-D3, Homeobox protein Hox-41, Homeobox protein MH-19, Hoxd3, Hox-41, Hoxd-3 |
| Entrez GeneID | 15434 |
| WB Predicted band size | 46.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This Mouse Hoxd3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-338 amino acids from the C-terminal region of mouse Hoxd3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于Mouse Hoxd3抗体的相关文献示例(内容为模拟概括，仅供参考)：
1. **文献名称**：*Hoxd3 is required for anterior-posterior patterning of the limb skeleton*
**作者**：Davis AP, et al.
**摘要**：研究利用Hoxd3特异性抗体，通过免疫组化分析发现Hoxd3蛋白在小鼠胚胎肢芽前部区域特异性表达，其缺失导致肢体骨骼发育异常，揭示了Hoxd3在肢体极性建立中的关键作用。
2. **文献名称**：*Regulation of Hoxd3 expression in neural crest cells during craniofacial development*
**作者**：Trainor PA, Krumlauf R
**摘要**：通过Western blot和免疫荧光技术，验证了Hoxd3抗体在小鼠颅神经嵴细胞中的特异性标记，证明Hoxd3通过调控细胞迁移相关基因影响颅面形态发生。
3. **文献名称**：*Antibody-based mapping of Hoxd3 functional domains in somitogenesis*
**作者**：Zakany J, Duboule D
**摘要**：该研究开发了针对小鼠Hoxd3不同结构域的多克隆抗体，结合ChIP-seq技术定位其DNA结合位点，揭示了Hoxd3在体节分节过程中通过调控下游靶基因参与轴向模式形成。
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**备注**：以上文献信息为示例性模拟，实际引用需以具体实验论文为准。建议通过PubMed或Web of Science以关键词"Mouse Hoxd3 antibody"+"gene function"检索近期文献。部分经典研究可能发表于1990-2010年间(如Developmental Biology等期刊)。
The mouse Hoxd3 antibody is a specialized tool used to detect and study the Hoxd3 protein, a member of the Hox gene family critical in regulating embryonic development. Hox genes, including Hoxd3. encode transcription factors that govern body patterning along the anterior-posterior axis by controlling cell differentiation and tissue morphogenesis. Specifically, Hoxd3 is involved in developmental processes such as hindbrain segmentation, cervical vertebral formation, and neural crest cell migration. It is expressed in spatially restricted domains during embryogenesis, influencing the identity of cells and structures in the developing embryo.
This antibody is typically generated in rabbits or other host species using immunogenic peptides or recombinant proteins derived from the mouse Hoxd3 sequence. It is validated for applications like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to visualize Hoxd3 expression in tissues or cultured cells. Researchers rely on its specificity to distinguish Hoxd3 from other Hox proteins, which share conserved homeodomains but exhibit distinct functional roles. Validation often includes knockout controls or siRNA knockdown to confirm minimal cross-reactivity.
Hoxd3 antibodies are pivotal in developmental biology studies, particularly for investigating axial patterning defects, congenital malformations, or roles in cancer metastasis, where Hoxd3 dysregulation has been implicated. Their use helps unravel molecular mechanisms underlying embryogenesis and disease models.
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