| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Phosphorylase b kinase gamma catalytic chain, liver/testis isoform, PHK-gamma-LT, PHK-gamma-T, Phosphorylase kinase subunit gamma-2, Phkg2 |
| Entrez GeneID | 68961 |
| WB Predicted band size | 46.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Mouse Phkg2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-29 amino acids from the N-terminal region of mouse Phkg2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse Phkg2 (N-term)抗体的模拟参考文献示例(注：以下内容为虚构示例，实际文献需通过学术数据库检索)：
1. **文献名称**：Phosphorylase kinase gamma 2 (Phkg2) regulates hepatic glycogen metabolism in mice
**作者**：Smith J, et al.
**摘要**：本研究通过Western blot和免疫组化分析，利用Phkg2 N端特异性抗体，揭示了Phkg2在肝脏糖原分解中的关键作用。实验表明，Phkg2缺失小鼠表现出糖原累积异常，提示其通过调节磷酸化酶活性影响代谢稳态。
2. **文献名称**：Characterization of Phkg2 knockout mouse model using N-terminal specific antibodies
**作者**：Lee H, et al.
**摘要**：作者开发了Phkg2基因敲除小鼠，并通过N端抗体验证了蛋白表达缺失。研究发现，Phkg2缺失导致骨骼肌糖原磷酸化酶活性降低，证实其在能量代谢中的必要性。
3. **文献名称**：Dynamic phosphorylation of Phkg2 during muscle contraction revealed by phospho-specific antibodies
**作者**：Garcia R, et al.
**摘要**：该研究利用Phkg2 N端抗体结合磷酸化位点检测，发现运动诱导的Phkg2磷酸化调控其与糖原磷酸化酶的相互作用，为肌肉收缩的能量供应机制提供了新见解。
4. **文献名称**：Impaired synaptic plasticity in Phkg2-deficient mice: Evidence from hippocampal studies
**作者**：Wang Y, et al.
**摘要**：通过免疫沉淀和Phkg2 N端抗体检测，研究发现海马区Phkg2表达与突触可塑性相关，缺失该蛋白导致小鼠学习记忆能力下降，提示其在中枢神经系统中的潜在功能。
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**建议**：实际文献可通过PubMed、Google Scholar等平台，以关键词“Phkg2 antibody”、“Phosphorylase kinase gamma 2 mouse”或抗体编号(如AB123)检索。部分抗体厂商(如Abcam、Sigma)的产品页面也会列出相关引用文献。
The Mouse Phkg2 (N-term) antibody is designed to detect the N-terminal region of phosphorylase kinase gamma 2 (PhKG2), a catalytic subunit of the phosphorylase kinase (PhK) complex. PhKG2 plays a critical role in glycogen metabolism by activating glycogen phosphorylase, which drives the breakdown of glycogen to glucose-1-phosphate during energy demand. This enzyme is highly expressed in tissues with high glycogen turnover, such as liver, muscle, and brain, and is regulated by calcium/calmodulin signaling and phosphorylation.
The antibody is commonly used in research to study PhKG2 expression, localization, and function in physiological and pathological contexts, including metabolic disorders, muscle diseases, and neurological conditions. Its specificity for the N-terminal region allows targeted detection of intact or truncated forms of PhKG2. aiding in investigations of post-translational modifications or mutations. Validated applications include Western blotting, immunohistochemistry, and immunofluorescence, often in murine models due to its specificity for mouse PhKG2. Researchers utilize this tool to explore PhKG2’s role in cellular energy regulation, glycogen storage diseases, and its potential interaction with other metabolic pathways. Proper controls, such as Phkg2-knockout samples, are recommended to confirm antibody specificity in experimental setups.
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