| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Serine/threonine-protein kinase Nek5, Never in mitosis A-related kinase 5, NimA-related protein kinase 5, Nek5 |
| Entrez GeneID | 330721 |
| WB Predicted band size | 71.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Nek5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 525-552 amino acids from the C-terminal region of mouse Nek5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse Nek5抗体的3篇参考文献，涵盖其应用和功能研究：
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1. **文献名称**：*Nek5 regulates cell cycle progression in mouse embryonic fibroblasts through kinase-dependent mechanisms*
**作者**：Smith A, et al.
**摘要**：该研究通过Western blot和免疫荧光技术，利用特异性Mouse Nek5抗体揭示Nek5在小鼠胚胎成纤维细胞周期G1/S转换中的调控作用，表明其缺失导致细胞周期停滞。
2. **文献名称**：*Characterization of a polyclonal antibody against mouse Nek5 and its application in detecting protein expression in neural tissues*
**作者**：Chen L, et al.
**摘要**：作者开发了一种兔源多克隆抗体，用于检测小鼠脑组织中的Nek5蛋白。通过免疫组化和免疫沉淀验证抗体特异性，发现Nek5在海马神经元中高表达，提示其潜在神经功能。
3. **文献名称**：*Nek5 deficiency in mice leads to cardiac dysfunction via dysregulated mitochondrial dynamics*
**作者**：Yamamoto K, et al.
**摘要**：研究利用基因敲除小鼠和Nek5抗体进行心脏组织分析，发现Nek5缺失导致线粒体动态异常和心功能不全，抗体验证了蛋白表达下调与病理表型的关联。
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**备注**：Nek5相关研究相对较少，上述文献为模拟示例，实际文献需通过数据库(如PubMed、Google Scholar)检索确认。建议结合关键词“Nek5 antibody mouse”或“Nek5 kinase murine”进一步查找。
The Mouse Nek5 antibody is a research tool designed to specifically detect and analyze Nek5 (NIMA-related kinase 5), a serine/threonine kinase belonging to the NIMA (Never in mitosis gene A) kinase family. Nek5 is involved in regulating cell cycle progression, microtubule dynamics, and cellular responses to DNA damage. It shares structural homology with other Nek kinases, featuring an N-terminal catalytic kinase domain and a C-terminal regulatory region. While its precise biological functions remain under investigation, Nek5 has been implicated in maintaining centrosome integrity, promoting cell survival, and influencing differentiation processes in certain tissues.
This antibody is typically generated using recombinant proteins or synthetic peptides corresponding to conserved regions of mouse Nek5. ensuring specificity for mouse-derived samples. It enables researchers to study Nek5 expression, localization, and protein interactions through techniques like Western blotting, immunofluorescence, and immunoprecipitation. Mouse Nek5 antibodies are particularly valuable in models exploring tissue development, cancer biology (due to Nek5's potential role in tumorigenesis), and neurodegenerative diseases, where cell cycle dysregulation may contribute to pathology. Recent studies also suggest links between Nek5 and mitochondrial function, expanding its relevance to metabolic disorders. Validation of antibody specificity through knockout controls or siRNA knockdown is critical, as cross-reactivity with other Nek family members can occur.
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