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Rabbit Polyclonal USP43 Antibody

  • 中文名: USP43抗体
  • 别    名: Ubiquitin carboxyl-terminal hydrolase 43, Deubiquitinating enzyme 43, Ubiquitin thioesterase 43, Ubiquitin-specific-processing protease 43, USP43
货号: IPDX31436
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/500 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesUbiquitin carboxyl-terminal hydrolase 43, Deubiquitinating enzyme 43, Ubiquitin thioesterase 43, Ubiquitin-specific-processing protease 43, USP43
Entrez GeneID124739
WB Predicted band size122.8kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis USP43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 900-928 amino acids from the C-terminal region of human USP43.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于USP43抗体的3篇示例文献(内容为模拟概括,具体文献需通过学术数据库验证):

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1. **文献名称**:*USP43 regulates DNA damage repair through deubiquitinating 53BP1 in cancer cells*

**作者**:Li X, et al.

**摘要**:本研究利用USP43特异性抗体,通过免疫共沉淀和免疫荧光技术,发现USP43通过去泛素化修饰调控53BP1的稳定性,影响乳腺癌细胞的DNA损伤修复能力,提示其在癌症治疗中的潜在靶点作用。

2. **文献名称**:*Characterization of a novel monoclonal antibody against USP43 for immunohistochemical analysis*

**作者**:Wang Y, et al.

**摘要**:作者开发了一种针对USP43的单克隆抗体,验证了其在人组织样本中的特异性和敏感性。该抗体成功应用于结直肠癌组织的免疫组化分析,显示USP43表达与患者预后负相关。

3. **文献名称**:*USP43 modulates hepatic lipid metabolism via SREBP1 deubiquitination*

**作者**:Zhang R, et al.

**摘要**:通过Western blot和免疫沉淀实验(使用USP43抗体),研究发现USP43通过去泛素化SREBP1调控肝脏脂质代谢,为代谢综合征机制提供了新见解。

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**备注**:以上为示例性内容,实际文献需通过PubMed、Google Scholar等平台以关键词“USP43 antibody”或“USP43 function”检索。若研究领域较新,建议联系相关领域作者或关注预印本平台(如bioRxiv)。

背景信息

The USP43 antibody targets ubiquitin-specific protease 43 (USP43), a member of the deubiquitinating enzyme (DUB) family that regulates protein stability by removing ubiquitin chains from substrates. USP43 contains conserved catalytic domains typical of USP-family proteases, including the cysteine and histidine boxes critical for its enzymatic activity. While its biological roles are less characterized compared to other USPs, emerging studies suggest USP43 may influence cellular processes such as DNA repair, epigenetic regulation, and cancer progression. For example, USP43 has been linked to histone H2B deubiquitination, potentially modulating chromatin structure and gene expression. Dysregulation of USP43 has been observed in hepatocellular carcinoma, breast cancer, and colorectal cancer, where it may act as either an oncogene or tumor suppressor depending on context. USP43 antibodies are essential tools for detecting protein expression, localization, and post-translational modifications in research. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate USP43's interaction networks, substrate specificity, and functional roles in disease models. Validated USP43 antibodies often undergo specificity testing (e.g., knockout cell line validation) to ensure reliable results. Ongoing research aims to clarify USP43's regulatory mechanisms and therapeutic potential in precision medicine.

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