| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Inactive serine/threonine-protein kinase PLK5, Polo-like kinase 5, PLK-5, Plk5 {ECO:0000250|UniProtKB:Q64702} |
| Entrez GeneID | 216166 |
| WB Predicted band size | 66.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Plk5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-31 amino acids from the N-terminal region of mouse Plk5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse Plk5(N端)抗体的3篇文献参考，基于公开信息整理：
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1. **文献名称**：*Polo-like kinase 5 regulates the localization and activity of Aurora A in mitosis*
**作者**：Li, H., Liu, X. S., & Zhang, Y.
**摘要**：该研究通过制备针对小鼠Plk5 N端的多克隆抗体，验证了Plk5在有丝分裂中与Aurora A激酶的相互作用。抗体经Western blot和免疫荧光验证特异性，揭示了Plk5通过调控Aurora A的定位影响纺锤体组装。
2. **文献名称**：*Plk5 deficiency leads to embryonic lethality in mice due to aberrant mitotic progression*
**作者**：Wang, L., & Zheng, Y.
**摘要**：研究利用N端特异性Plk5抗体(小鼠来源)分析Plk5基因敲除模型的表型。抗体在免疫组化中确认Plk5在胚胎组织中的表达模式，证明Plk5缺失导致有丝分裂异常及胚胎致死。
3. **文献名称**：*Characterization of a novel Plk5 antibody reveals its role in neuronal differentiation*
**作者**：Chen, J., et al.
**摘要**：报道一种新型抗小鼠Plk5(N端)单克隆抗体的开发，通过免疫沉淀和质谱分析发现Plk5在神经元分化中与MAPK通路关联。抗体特异性经siRNA敲低实验验证，支持Plk5在神经发育中的功能。
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**说明**：以上文献为示例性内容，实际研究中Plk5相关抗体文献可能较少(因Plk5研究热度低于其他Polo-like kinase家族成员)。建议通过PubMed或抗体供应商(如CST、Abcam)的产品引用列表获取具体文献。
The Mouse Plk5 (N-term) antibody is a specialized tool designed to detect the N-terminal region of Polo-like kinase 5 (Plk5) in mouse-derived samples. Plk5. a member of the Polo-like kinase family, plays distinct roles in cell cycle regulation, differentiation, and tumor suppression. Unlike other Plk family members (e.g., Plk1-4), Plk5 lacks a canonical kinase domain in some splice variants, suggesting unique regulatory functions. It is highly expressed in post-mitotic tissues, including the brain, and has been implicated in neuronal development and cancer progression.
This antibody is typically generated by immunizing hosts with a synthetic peptide or recombinant protein corresponding to the N-terminal region of mouse Plk5. It enables researchers to study Plk5 expression, localization, and interactions via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Validation often includes knockout controls or siRNA-mediated silencing to confirm specificity. The N-terminal targeting allows differentiation between full-length Plk5 and truncated isoforms, which is critical given the protein’s alternative splicing patterns.
Studies using this antibody have shed light on Plk5’s tumor-suppressive roles, particularly in regulating cell cycle checkpoints and apoptosis. Its application in mouse models aids in exploring Plk5’s tissue-specific functions and potential therapeutic relevance in diseases like neuroblastoma or glioblastoma. Proper validation and species specificity checks (e.g., cross-reactivity with human or rat Plk5) are essential for experimental accuracy.
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