| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA-directed RNA polymerase II subunit RPB11-a, RNA polymerase II subunit B11-a, RPB11a, DNA-directed RNA polymerase II subunit J-1, RNA polymerase II 133 kDa subunit, POLR2J, POLR2J1 |
| Entrez GeneID | 5439 |
| WB Predicted band size | 13.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This POLR2J antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the C-terminal region of human POLR2J. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于POLR2J抗体的模拟参考文献示例(注：内容为虚构，仅用于示例格式)：
1. **文献名称**：*POLR2J as a novel biomarker in hepatocellular carcinoma: Antibody validation and clinical implications*
**作者**：Chen L, et al.
**摘要**：本研究利用特异性POLR2J抗体，通过免疫组化技术发现POLR2J在肝癌组织中显著高表达，提示其可能作为肝癌诊断标志物，并探讨了其在肿瘤细胞异常增殖中的作用机制。
2. **文献名称**：*Development of a high-affinity monoclonal antibody targeting human RNA polymerase II subunit POLR2J*
**作者**：Smith K, et al.
**摘要**：文章报道了一种新型POLR2J单克隆抗体的开发，通过ELISA和Western blot验证其高特异性和灵敏度，适用于染色质免疫沉淀(ChIP)等表观遗传学研究。
3. **文献名称**：*POLR2J-mediated transcriptional dysregulation in neurodegenerative diseases*
**作者**：Park J, et al.
**摘要**：研究利用POLR2J抗体分析阿尔茨海默病患者脑组织样本，发现POLR2J与RNA聚合酶II活性异常相关，可能通过影响转录延伸参与神经退行性病变。
(注：以上文献为示例性虚构内容，实际研究中请通过学术数据库查询真实文献。)
The POLR2J antibody targets the POLR2J protein, a key subunit of RNA polymerase II (Pol II), the enzyme responsible for transcribing protein-coding genes in eukaryotic cells. POLR2J, also known as RPB11. is part of the core Pol II complex, which consists of 12 subunits and plays a central role in mRNA synthesis. This subunit contributes to the structural integrity of Pol II and is involved in coordinating interactions with transcription factors, regulatory proteins, and nucleic acids during transcription initiation and elongation.
POLR2J antibodies are widely used in research to study Pol II assembly, transcription mechanisms, and gene expression regulation. They are essential tools in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to visualize Pol II localization, assess its binding to DNA, or investigate transcriptional dysregulation in diseases. Aberrant Pol II activity, including mutations or altered expression of its subunits, has been linked to cancers, neurological disorders, and developmental defects, making POLR2J a subject of interest in both basic and clinical research.
Commercial POLR2J antibodies are typically raised in hosts like rabbits or mice, using immunogenic peptides derived from conserved regions of the human POLR2J protein. Validation often includes testing for specificity in knockout cell lines or tissues. Researchers rely on these antibodies to explore transcriptional dynamics, cellular stress responses, and the effects of therapeutic agents targeting transcription in disease models.
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