| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Lck-interacting transmembrane adapter 1, Lck-interacting membrane protein, Lck-interacting molecule, LIME1, LIME |
| Entrez GeneID | 54923 |
| WB Predicted band size | 31.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This LIME1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 190-219 amino acids from the C-terminal region of human LIME1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于LIME1抗体的模拟参考文献示例(注：部分内容基于文献背景模拟，建议通过学术数据库核实具体信息)：
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1. **文献名称**：*LIME1 modulates T-cell activation by regulating Lck membrane localization*
**作者**：Brumbaugh, J. et al.
**摘要**：本研究利用LIME1特异性抗体，揭示了LIME1作为跨膜适配蛋白通过调控Lck激酶的膜定位，参与T细胞受体(TCR)信号通路的早期激活，为T细胞免疫应答机制提供了新见解。
2. **文献名称**：*Structural characterization of LIME1 phosphotyrosine motifs using monoclonal antibodies*
**作者**：Sato, K. & Takeda, H.
**摘要**：通过开发针对LIME1磷酸化酪氨酸表位的单克隆抗体，作者解析了其与下游信号分子(如Grb2)的结合机制，证实LIME1在炎症反应中的动态修饰过程。
3. **文献名称**：*LIME1 antibody-based detection in B-cell malignancies*
**作者**：Müller, R. et al.
**摘要**：研究利用抗LIME1抗体进行流式细胞术和免疫组化分析，发现LIME1在部分B细胞淋巴瘤中异常高表达，提示其可能作为血液肿瘤的新型生物标志物。
4. **文献名称**：*LIME1 knockout mice reveal its role in autoimmune regulation*
**作者**：Chen, L. et al.
**摘要**：通过LIME1抗体检测基因敲除小鼠模型，发现LIME1缺失导致调节性T细胞(Treg)功能缺陷，加剧实验性自身免疫性脑脊髓炎(EAE)，表明其在免疫耐受中的关键作用。
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建议通过PubMed或Google Scholar以关键词“LIME1 antibody”、“LIME1 signaling”或“Lck interacting protein”检索最新文献以获取准确信息。
LIME1 (Lck-interacting membrane protein 1) is a transmembrane adaptor protein primarily expressed in immune cells, notably T lymphocytes and natural killer (NK) cells. It plays a critical role in regulating T-cell receptor (TCR)-mediated signaling by facilitating the assembly of signaling complexes at the immune synapse. Structurally, LIME1 contains a short extracellular domain, a transmembrane region, and a cytoplasmic tail with multiple tyrosine phosphorylation sites. Upon TCR activation, LIME1 interacts with the Src-family kinase Lck, promoting phosphorylation of downstream effectors like ZAP-70 and LAT, which propagate signals for T-cell activation, proliferation, and cytokine production.
LIME1 antibodies are essential tools for studying its expression, localization, and function in immune responses. These antibodies are commonly used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to detect LIME1 in cell lysates or tissue samples. Researchers also employ them to investigate dysregulated LIME1 expression in autoimmune diseases (e.g., rheumatoid arthritis) and hematologic malignancies, where aberrant TCR signaling may contribute to pathogenesis. Some antibodies specifically target phosphorylated tyrosine residues to assess LIME1 activation status. Validation often includes knockout cell lines or siRNA-mediated silencing to confirm specificity. As LIME1's role in immune regulation becomes clearer, its antibodies remain pivotal for elucidating mechanisms underlying T-cell dysfunction and therapeutic targeting.
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