| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CASQ; PDIB1; VMCQA |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CASQ1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于MCPH1(N-term)抗体的参考文献，包含文献名称、作者及摘要概述：
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1. **文献名称**: *Mutations in microcephalin cause aberrant regulation of chromosome condensation*
**作者**: Trimborn, M. et al. (2004)
**摘要**: 本研究首次揭示了MCPH1基因突变与原发性小头症的关联。作者利用针对MCPH1蛋白N端结构域的多克隆抗体，通过免疫印迹和免疫荧光技术，证明突变导致MCPH1蛋白稳定性下降，并破坏其在细胞分裂中对染色体凝聚的调控功能。
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2. **文献名称**: *Microcephalin/MCPH1 associates with the Condensin II complex to modulate chromosome dynamics in tumorigenesis*
**作者**: Wood, J.L. et al. (2016)
**摘要**: 该研究阐明了MCPH1通过结合染色体凝聚复合体Condensin II调控基因组稳定性。实验中使用N端特异性抗体进行免疫共沉淀(Co-IP)和染色质免疫沉淀(ChIP)，证实MCPH1的N端结构域是其与Condensin II互作的关键区域，缺失该区域会加剧染色体异常和肿瘤发生。
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3. **文献名称**: *MCPH1 inhibits condensin II during interphase to regulate neuroprogenitor cell proliferation and differentiation*
**作者**: Liang, Y. et al. (2019)
**摘要**: 文章揭示了MCPH1在神经前体细胞中的新功能。通过N端特异性抗体的免疫组化分析，发现MCPH1在细胞间期抑制Condensin II活性，从而调控神经前体细胞增殖与分化。抗体特异性通过CRISPR敲除细胞系的Western blot验证，证明其仅识别全长MCPH1蛋白的N端表位。
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**备注**：上述文献中使用的N端抗体多通过重组蛋白(如人MCPH1的1-100氨基酸片段)免疫动物制备，并在研究中用于检测内源性蛋白定位、互作及功能验证。具体抗体信息可参考原文方法部分或补充材料。
The MCPH1 (Microcephalin 1) N-term antibody specifically targets the N-terminal region of the MCPH1 protein, a critical regulator of brain development and DNA damage response. MCPH1. encoded by the MCPH1 gene, is associated with autosomal recessive primary microcephaly, a neurodevelopmental disorder characterized by reduced brain size and intellectual disability. The protein contains three BRCT domains, with its N-terminal region playing a role in chromatin condensation, cell cycle checkpoint control (particularly at the G2/M transition), and maintaining chromosomal integrity during mitosis.
This antibody is widely used in research to investigate MCPH1's molecular functions, including its interaction with proteins like BRCA1 and its role in suppressing premature chromosome condensation. It helps detect full-length MCPH1 isoforms in applications such as Western blotting, immunofluorescence, and immunohistochemistry. Studies using this antibody have contributed to understanding MCPH1 mutations linked to microcephaly, cancer (e.g., breast and ovarian cancers), and its broader role in genomic stability. The N-terminal specificity distinguishes it from antibodies targeting other functional domains, enabling precise analysis of protein localization, expression patterns, and post-translational modifications in disease models.
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