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Rabbit Polyclonal MouseMap2k5 Antibody

  • 中文名: Mouse Map2k5抗体
  • 别    名: Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MAPK/ERK kinase 5, MEK 5, Map2k5, Mek5, Mkk5, Prkmk5
货号: IPDX31407
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesDual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MAPK/ERK kinase 5, MEK 5, Map2k5, Mek5, Mkk5, Prkmk5
Entrez GeneID23938
WB Predicted band size50.1kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenThis Mouse Map2k5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 385-412 amino acids from the C-terminal region of mouse Map2k5.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是几篇关于Mouse Map2k5(MEK5)抗体的示例参考文献(注:以下为虚构示例,实际文献需通过学术数据库检索验证):

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1. **"Characterization of a novel monoclonal antibody targeting Map2k5 in murine models"**

*Authors: Tanaka H, et al.*

摘要:本研究开发了一种针对小鼠Map2k5蛋白的单克隆抗体,验证了其在Western blot和免疫组织化学中的特异性。实验证明该抗体可特异性识别小鼠心脏和脑组织中的Map2k5.并在Map2k5基因敲除模型中显示无交叉反应性。

2. **"Map2k5 signaling in cardiac hypertrophy: Insights from antibody-based inhibition studies"**

*Authors: Smith JL, et al.*

摘要:通过使用小鼠Map2k5特异性抗体,研究发现Map2k5在压力超负荷诱导的心肌肥厚中显著激活。抗体阻断实验表明,抑制Map2k5可减轻心肌细胞肥大表型,提示其作为治疗靶点的潜力。

3. **"Dysregulation of MEK5/ERK5 pathway in neurodegenerative disorders: Evidence from mouse models"**

*Authors: Chen R, et al.*

摘要:利用Map2k5抗体分析阿尔茨海默病模型小鼠脑组织,发现MEK5/ERK5通路活性降低与tau蛋白异常磷酸化相关。抗体染色显示Map2k5在神经元中的定位与疾病进展相关。

4. **"Validation of commercial Map2k5 antibodies for murine tissue-specific applications"**

*Authors: Gonzalez P, et al.*

摘要:系统性评估了多种市售小鼠Map2k5抗体的灵敏度与特异性,推荐适用于不同实验场景(如流式细胞术、免疫沉淀)的抗体,并提供了组织裂解液制备的优化方案。

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**建议**:实际文献可通过PubMed或Google Scholar以关键词“MEK5 antibody mouse”“Map2k5 signaling”或“MEK5/ERK5 pathway”检索,并优先选择近5年内的研究以获取最新进展。

背景信息

The Mouse MAP2K5 antibody is a tool used to detect and study mitogen-activated protein kinase kinase 5 (MAP2K5), also known as MEK5. a serine/threonine kinase in the MAPK signaling pathway. MAP2K5 functions as a dual-specificity kinase that activates ERK5 (MAPK7) by phosphorylating its threonine and tyrosine residues. This pathway regulates cellular responses such as proliferation, differentiation, and survival, with ERK5 playing roles in angiogenesis, stress responses, and development. The MAP2K5 antibody is commonly employed in Western blotting, immunohistochemistry, and immunoprecipitation to investigate endogenous MAP2K5 expression, localization, and interactions in mouse tissues or cell lines. Its specificity is validated through knockout controls or siRNA-mediated silencing. MAP2K5 is structurally characterized by a catalytic domain and regulatory motifs, and its activation depends on upstream signals from MEKK2/3. Dysregulation of the MAP2K5-ERK5 axis is implicated in cancer, cardiovascular diseases, and neurodegenerative disorders. Researchers use this antibody to explore MAP2K5's role in signaling cascades, disease mechanisms, and therapeutic targeting. Cross-reactivity with other MEK family members should be ruled out during experimental design. Commercial variants often differ in host species (e.g., rabbit, mouse), clonality (monoclonal/polyclonal), and epitope recognition regions.

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