| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial, Pyruvate dehydrogenase kinase isoform 2, PDH kinase 2, Pdk2 |
| Entrez GeneID | 18604 |
| WB Predicted band size | 46.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Mouse Pdk2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78-107 amino acids from the N-terminal region of mouse Pdk2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse PDK2 (N-term)抗体的3篇参考文献示例(注：以下内容为模拟示例，实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*"Characterization of PDK2 Isoform-Specific Antibodies in Metabolic Regulation Studies"*
**作者**：Smith A, et al.
**摘要**：本研究开发并验证了针对小鼠PDK2蛋白N端结构域的多克隆抗体。通过Western blot和免疫组化证实该抗体特异性识别PDK2.并应用于高脂饮食模型中，发现PDK2在肝脏中的表达显著上调，提示其与胰岛素抵抗相关。
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2. **文献名称**：*"Role of PDK2 in Cardiac Energy Metabolism: Insights from Knockout Mice"*
**作者**：Zhang L, et al.
**摘要**：利用PDK2 (N-term)抗体检测心脏组织中PDK2的蛋白水平，结合基因敲除模型，发现PDK2缺失导致丙酮酸脱氢酶活性增强，促进葡萄糖氧化，从而改善心力衰竭模型的能量代谢异常。
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3. **文献名称**：*"Antibody-Based Detection of PDK2 in Pancreatic β-Cells and Its Implications for Diabetes"*
**作者**：Tanaka K, et al.
**摘要**：通过免疫荧光和流式细胞术，使用PDK2 (N-term)抗体揭示其在胰岛β细胞中的亚定位。研究发现PDK2通过调控线粒体代谢影响胰岛素分泌，为糖尿病治疗提供了新靶点。
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**提示**：建议通过PubMed、Google Scholar等平台，以关键词“PDK2 antibody N-terminal mouse”检索最新文献，或参考抗体供应商(如Abcam、Cell Signaling Technology)官网提供的引用文献。
The Mouse Pdk2 (N-term) antibody is designed to detect the N-terminal region of pyruvate dehydrogenase kinase 2 (PDK2), a key regulatory enzyme in cellular metabolism. PDK2 belongs to the PDK family (PDK1-4) and phosphorylates the pyruvate dehydrogenase complex (PDC), inhibiting its activity and modulating the transition between glycolysis and oxidative phosphorylation. This regulation is critical under conditions such as hypoxia or nutrient stress, where PDK2 helps redirect metabolic flux to support cell survival. Dysregulation of PDK2 has been implicated in metabolic disorders, cancer (via the Warburg effect), and neurodegenerative diseases.
The antibody specifically targets the N-terminal epitope of mouse PDK2. enabling researchers to study its expression, localization, and function in murine models. It is commonly used in techniques like Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP) to investigate PDK2's role in tissue-specific metabolic adaptations, disease mechanisms, or therapeutic responses. Validated for specificity and sensitivity, the antibody aids in distinguishing PDK2 from other PDK isoforms, which share structural similarities but differ in expression patterns and regulatory roles. Its application extends to studies on insulin resistance, mitochondrial dysfunction, and metabolic reprogramming in cancer, offering insights into PDK2 as a potential therapeutic target.
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